TNF-α、sICAM-1在慢性间歇低氧条件下大鼠牙周炎发病机制中的作用

摘要

Objective:To investigate the expression of tumor necrosis factor-α(TNF-α)and soluble intercellular adhesion molecule -1(sICAM-1)in blood serum and gingival tissue in rat periodontitis model in normoxia and chronic intermittent hypoxia environment. Methods:32 male SD rats were randomly divided into 4 groups(n =8):normoxia control group(group A),normoxia periodontitis group(group B),hypoxic control group(group C)and hypoxia periodontitis group(group D).The periodontitis models were estab-lished by ligating the bilateral maxillary second molar and raised by periodontitis diet.The rats in hypoxia groups were raised under chronic intermittent hypoxia environment,while those in normoxia groups were raised under normoxia environment.After 8 weeks, plaque index(PLI),bleeding index(BI)and attachment lost(AL)were measured,TNF-αand sICAM-1 concentrations in blood serum and gingival tissue were measured by ELISA.Results:TNF-αand sICAM-1 concentrations in blood serum and gingival tissue in group A were higher than those in group B,C(P <0.05);in group D were lower than in group B and C(P <0.05).TNF-αand sICAM-1 levels in blood serum and gingival tissue were positively correlated with AL(P <0.05).Conclusion:In chronic intermittent hypoxic environment TNF-αand sICAM-1 may aggravate periodontitis,and promote the inflammatory response to peripheral vascular system.%目的：探讨慢性间歇低氧和常氧条件下大鼠血清和牙龈组织中肿瘤坏死因子-α（TNF-α）、可溶性细胞间黏附分子-1（sICAM-1）在牙周炎发病机制中的作用。方法：将32只雄性 SD 大鼠随机分为4组（n ＝8）：常氧对照组（A 组）、常氧牙周炎组（B 组）、低氧对照组（C 组）、低氧牙周炎组（D 组）。采用正畸丝结扎双侧上颌第二磨牙和牙周炎食谱的方法建立牙周炎模型，常氧组和低氧组分别在常氧和模拟阻塞性呼吸暂停低通气综合征条件下饲养8周，检测各组动物牙周组织各项临床指标，用 ELISA 法检测动物血清和牙龈组织中 TNF-α、sICAM-1表达。结果：低氧牙周炎组中 TNF-α、sICAM-1浓度显著高于其余各组 P ＜0．05。血清和牙龈组织中 TNF-α、sICAM-1与附着丧失（AL）成正相关（P ＜0．05）。结论：慢性间歇低氧条件下 TNF-α、sICAM-1在牙周组织中的表达水平升高，加重了牙周炎症破坏程度，同时促使炎症反应向外周血管扩散。