成骨细胞在激光立体成形多孔钛表面的附着、增殖及 ALP 活性

摘要

目的：评价激光立体成形（LSF）的多孔钛对成骨细胞的生物相容性。方法：将 SD 大鼠成骨细胞接种于材料表面进行培养，对不同培养时间点成骨细胞在材料表面的黏附、增殖及 ALP 活性进行检测，并观察细胞形态。以经喷砂粗化处理的致密纯钛作为对照，用 SPSS 对试验结果进行统计分析。结果：培养2、3 h 时实验组细胞贴附率高于对照组（P ＜0．05），4、7 d时细胞增殖和 ALP 活性明显高于对照组（P ＜0．05）。结论：激光成形的多孔钛材料具有良好的骨组织生物相容性。%Objective:To study the biocompatibility of porous Ti processed by laser solid forming(LSF)with osteoblasts.Methods:SD rat osteoblasts were seeded on porous Ti surface processed by LSF.Adhesion,proliferation and ALP activity of the osteoblasts were measured at different time,and all samples were observed under SEM.Dense sand-blasted Ti sheets were used as the controls.All data were statistically analyzed.Results:Adhered cells on porous Ti were more than those on nonporous Ti sheets after 2 and 3 hours of culture(P <0.05).At 4 and 7 days,cell proliferation and ALP activity were significantly higher on porous Ti than those on the controls(P <0.05).Conclusion:Porous Ti processed by LSF possesses good biocompatibility with osteoblasts.