完善根管充填后治疗失败感染根管中粪肠球菌的活菌研究

摘要

目的：将叠氮溴化丙锭（PMA）与定量 PCR（qPCR）技术结合，用于计算根管治疗失败感染根管中粪肠球菌活菌数量。方法：选取34名根管治疗失败患者，提取感染根管内细菌 DNA，每个样本分成2份，一份经 PMA 处理（实验组），一份未用PMA 处理（对照组），然后通过 qPCR 方法检测样本中粪肠球菌的活菌数量和细菌总量。结果：20例患者（58．8％）细菌样本中检测出粪肠球菌。实验组和对照组 Ct 值分别为25．12±2．04和24．62±2．02（P ＝0．001）。结论：对于根管治疗失败感染根管内活菌的研究使用 PMA 结合定量 PCR 可能是一种有效的方法。%Objective:To analyse viable Enterococcus faecalis(E.faecalis)in root-well-filled teeth associated with failed endodontic treatment by using propidium monoazide (PMA)in combination with real time qPCR.Methods:Bacterial samples were extracted from 34 root-canal-treated teeth with post-treatment apical periodontitis.Each sample was separated into 2 different tubes.PMA was added to one of the tubes,and the other was left untreated.Then,DNA extraction and qPCR were performed.Results:E.faecalis was found in 20 of the 34 samples(58.8%).In PMA treated and none-treaten samples the Ct value of E.faecalis was 25.1 2 ±2.04 and 24.62 ± 2.02 respectively(P =0.001 ).Conclusion:PMA may be feasible in differentiating viable and dead Enterococcus faecalis cells.