3种应用于正畸支抗的金属材料的细胞毒性和遗传毒性检测

摘要

目的:初步评价应用于口腔正畸支抗种植体的金属材料:商业纯钛(cpTi),钛合金(Ti6Al4V),医用316L不锈钢的生物安全性.方法:参考国标GB/T16886.5-2003以及医疗行业标准YY/T0127.2-2009所规定的方法,采用体外细胞毒性实验,体外遗传毒性实验及急性全身毒性实验对3种材料进行测试.结果:cpTi、Ti6Al4V和平316L不锈钢100％浸提液的细胞毒性分别为0级、1级、2级;DNA OTM分别为2.16 ±0.09,9.84±1.78和10.79±0.39;细胞尾部DNA分别为(0.43±0.01)％、(0.92±0.43)％和(1.22±0.06)％.小鼠体内急性毒实验3种材料显示毒性体征,未引起小鼠体重变化.结论:cpTi生物安全性优于Ti6 Al4V和316L.%Objective:To evaluate the biological safety of commercial pure Ti (cpTi),Ti alloy Ti6Al4V and stainless steel 316L.Methods:According to the GB/T16886.5-2003 and YY/T0127.2-2009 standards,the extraction of the 3 materials was respectively prepared,cck-8 assay was used to test their cytotoxicity,single cell gel electrophoresis was used to test their genotoxicity,and in vivo test was used to test their acute systemic toxicity.Results:The cytotoxicity of 100％ extractions of 316L,Ti6Al4V and cpTi was graded to 2,1 and 0,the olive tal moment(OTM) 10.79 ±0.39,9.84 ± 1.78 and 0.92 ± 1.43,the percentage of tail DNA (1.22 ±0.06) ％,(0.92 ± 0.43) ％ and (0.43 ± 0.01) ％ respectively.No systemic toxicity and no body weight change was observed in the acute systemic toxicity test in mice.Conclusion:cpTi has better biologic safety than Ti6Al4V and 316L.