口腔鳞状细胞癌中Dickkopf-3基因的表达及甲基化状态

摘要

目的: 探索Dickkopf-3 (DKK-3) 基因的表达水平及其启动子区甲基化状态与口腔鳞癌发生的关系.方法: 应用PT-PCR 及MSP-PCR 法检测DKK-3 在51 例口腔鳞癌及正常口腔黏膜组织中的表达及其启动子区的甲基化状态,SPSS13. 0统计分析DKK-3 与临床病理特征间的关系.结果: DKK-3 在口腔鳞癌中的表达量低于对照组(t = - 12. 580,P＜0. 05) .DKK-3 启动子区在口腔鳞癌中的甲基化率高于对照组(χ2 = 19. 273,P＜ 0. 05) .发生甲基化的口腔鳞癌DKK-3 的表达量低于对照组(t = - 2. 817,P＜ 0. 05) .结论: DKK-3 表达下调及其启动子区的高甲基化状态是口腔鳞癌发病的重要机制之一,启动子区的高甲基化可能是导致DKK-3 转录沉默的主要原因.%Objective: To explore the expression of Dickkopf-3(DKK-3) mRNA in oral squamous cell carcinoma(OSCC) and the relationship between the promoter methylation status and the carcinogenesis of OSCC. Methods: The expression of DKK-3 gene in 51 cases of OSCC and corresponding normal mucosa tissue was detected by PT-PCR and methylation-specific PCR, respectively. The relationship between DKK-3 and the clinical pathological features of the patients was analyzed with SPSS 13. 0. Results: The expression level of DKK-3 in OSCC group was lower than that in the control(t =-12. 580, P＜ 0. 05). The methylation rate of DKK-3 gene promoter region in OSCC group was significantly higher than that in the control(χ2 = 19. 273, P＜ 0. 05). The mRNA expression level of DKK-3 gene in OSCC with methylation group was lower than that in the control(t =-2. 817, P＜ 0. 05). Conclusion: Down-regulation of DKK-3 gene expression and hypermethylation of promoter region are important mechanisms in the pathogenesis of OSCC. The hypermethylation of DKK-3 promoter may be the main cause of transcriptional silencing.