骨碎补总黄酮对高原RA继发OP患者血清MMP-3、CD200及IL-17的作用研究

摘要

目的 研究骨碎补总黄酮(total flavonoids of rhizoma drynariae,TFRD)对高原类风湿关节炎(rheumatoid arthritis,RA)继发骨质疏松(osteoporosis,OP)患者血清基质金属蛋白酶-3(matrix metalloproteinase-3,MMP-3)、CD200、白介素-17(interleukin-17,IL-17)的影响.方法 选取我院2012年6月至2016年6月收治的38例高原RA继发OP患者为研究对象,选取38例单纯高原RA患者做为对照组,研究组给予骨碎补总黄酮胶囊(每粒含骨碎补总黄酮180 mg,2粒/次,3/日,8周)治疗,组内所有患者均于治疗前和治疗后同一天抽取静脉血,采用蛋白印迹法(Western blotting)检测MMP-3、CD200和IL-17表达变化,以探寻高原RA继发OP的分子机制,为临床治疗提供参考依据.结果 与正常对照组比较,骨碎补总黄酮组治疗前患者血清MMP-3、IL-17表达升高(P<0.05),CD200表达降低(P<0.05);经骨碎补总黄酮治疗后,骨碎补总黄酮组患者血清MMP-3、IL-17表达降低(P<0.05),CD200表达升高(P<0.05).结论 在高原RA继发OP患者发病中MMP-3、CD200及IL-17可能起到重要作用,细胞因子直接或间接影响机体免疫功能和炎症反应,骨碎补总黄酮能有效抑制上述因子的不良作用.%Objective To study the diference of the Matrix metalloproteinase-3(MMP-3),CD200 and Interleukin-17 levels of the patients with plateau RA with osteoporosis patients before and after treatment with TFRD.Methods Tests on 38 patients before and after treatment with TFRD in RA with osteoporosis patients,the control group by administering TFRD (1 080 mg/d;after 8 week),all patients before treatment and after treatment for venous blood,using MMP-3,CD200 and IL-17 to detect the Western expression of blot.Results Compared with the control group,the MMP-3,IL-17 expression increased significantly and CD200 expression decreased in the former TFRD group of patients(P<0.05);after treatmen with TFRD,the MMP-3,IL-17 were decreased(P<0.05),the CD200 expression was increased significantly(P<0.05).Conclusion The MMP-3,CD200 and IL-17 may play an important role in the highlands of RA with OP patients,cytokines directly or indirectly affect immune function and inflammatory response,TFRD can effectively prevent osteoporosis patients degree of aggravation.