卵巢上皮性癌组织及血清中MICA的表达和含量及临床意义

摘要

Objective:To investigate the expression and content of major histocompatibility complex class I-related protein A (MICA) in epithelial ovarian cancer tissues and in serum of ovarian cancer. To explore clinical significance and the relationship between the expression of MICA and the epithelial ovarian cancer in the term of the different cell differentiation degree and histological type and clinical stages. Methods:The expressions of mMICA in 59 cases of ovarian cancer, 12 ovarian benign tumor and 15 normal ovarian tissues were detected by immunohistochemistry. The serum levels of soluble major histocompatibility complex class l-related protein A (sMICA) were assessed by MICA-specific enzyme-linked immunosorbent assay. Results:① The positive expression rate of mMICA in epithelial ovarian malignant and benign tumor tissues were 72.88% and 8.33%, the difference was statistically significant (P<0.01); The positive expression rate of mMICA in moderately differentiated ovary cancer tissues was 63.16% while 90.48% in the poorly; in stage Ⅰ～Ⅱ and stage Ⅲ-Ⅳ were 45.45% and 79.17% respectively,the difference was statistically significant (P<0.05), There was no mMICA expression in the normal ovary epithelial tissues. ②The serum levels of sMICA in epithelial ovarian malignant and benign tumor and health donors were 20.79 ±8.37 ng/ml, 9.98 ±1.75 ng/ml and 6.47 ± 1.69 ng/ml respectively,the difference was statistically significant (P<0.01). ③ The positive expression rate of mMICA and the serum level of sMICA in different tissue type of epithelial ovarian cancer was rnno statistically significant ( P>0. 05). Conclusions:The positive expression rate of mMICA and the serum levels of sMICA in epithelial ovarian cancer are correlated with the cell differentiation and clinical stage of o-varian cancer. The level of sMICA could be considered as one of symbol in auxiliary diagnosis and analysis the development of ovarian cancer.%目的:检测卵巢上皮性癌组织及血清中主要组织相容性复合体相关蛋白A(MICA)的表达和含量,探讨其与卵巢上皮性癌细胞分化程度、组织学类型、临床分期的关系及临床意义.方法:免疫组化SP法检测卵巢上皮性癌(59例)及卵巢上皮性良性肿瘤组织(12例)、正常卵巢上皮组织(15例)膜型主要组织相容性复合体相关蛋白A (mMICA)的表达；ELISA法检测卵巢上皮性癌、卵巢上皮性良性肿瘤及健康人(15例)血清可溶性主要组织相容性复合体相关蛋白A(sMICA)的含量.结果:①mMICA在卵巢上皮性癌和卵巢上皮性良性肿瘤组织表达阳性率分别为72.88％、8.33％,差异有高度统计学意义(P＜0.01).在卵巢上皮性癌低分化组和中分化组表达阳性率分别为90.48％、63.16％,Ⅰ～Ⅱ期组和Ⅲ～Ⅳ期组表达阳性率分别为45.45％、79.17％,差异均有统计学意义(P＜0.05).在正常卵巢上皮组织不表达.②血清中sMICA含量,卵巢上皮性癌20.79±8.37 ng/ml、卵巢上皮性良性肿瘤9.98±1.75 ng/ml、健康人6.47±1.69 ng/ml,差异有高度统计学意义(P＜0.01).在卵巢上皮性癌低分化组含量高于中分化组,Ⅲ～Ⅳ期组高于Ⅰ～Ⅱ期组,差异有统计学意义及高度统计学意义(P ＜0.05,P＜0.01).③在卵巢上皮性癌不同组织学类型的卵巢上皮组织中mMICA的表达阳性率及血清中sMICA含量比较,差异均无统计学意义(P＞0.05).结论:卵巢上皮性癌组织mMICA表达阳性率及血清中sMICA含量均较高,且均与卵巢上皮性癌细胞分化程度、临床分期有关.检测血清sMICA含量可能作为辅助诊断及评价卵巢上皮性癌病情的一个指标.