目的:检测血管紧张素Ⅱ受体样受体蛋白(Apelin/APJ)在子痫前期患者与正常孕妇胎盘组织中的表达差异.方法:随机选择2008年9月至2010年6月我院收治的轻度子痫前期患者20例(mPE组),重度子痫前期患者10例(sPE组),正常妊娠妇女20例(对照组),收集其胎盘组织.通过免疫组化方法检测胎盘组织中Apelin/APJ的表达水平.结果:Apelin/APJ主要表达部位为滋养细胞和血管内皮细胞胞膜、胞浆,间质细胞胞膜、胞浆散在表达；Apelin/APJ在mPE组及sPE组表达量及表达强度较对照组均降低,差异有统计学意义(P＜0.05),且sPE组胎盘组织中Apelin/APJ表达较mPE组明显降低,差异有统计学意义(P＜0.05).结论:正常妊娠胎盘组织中Apelin/APJ表达明显高于子痫前期,且随子痫前期病情加重而降低,Apelin/APJ的表达异常可能参与了子痫前期的发生和病理生理过程.%Objective:To compare the expression of Apelin/APJ in placentas from preeclamptic pregnant patients and normal pregnant women. Methods: Placentas were obtained from patients with mild preeclampsia (mPE,n=20), severe preclamptic (sPE,n = 10) and normal pregnant women (control group,n =20) from Sep. 2008 to Jun.2010 in Guangdong General Hospital. The Apelin/APJ expressions were measured by immunohistochemical assays. Results;The Apelin and APJ were localized in the cytoplasm and membrane of the trophoblast and capillaries of placenta. The expression of Apelin/APJ in the placentas of preeclamptic pregnant patients was significantly lower than that of control group. Apelin/APJ was down-regulated in mPE (0. 82 fold) and sPE groups(0. 46 fold) (P<0. 05) ,and the expression level was correlated to the severity of pre-eclampsia (P<0. 05). Conclusions: Expression of Apelin/APJ was significantly lower in placenta of pre-eclampsia,especially in severe pre-eclampsia,which suggested that Apelin/APJ might be involved in the occurrence and development of preeclampsia.
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