慢性阻塞性肺疾病患者血清泛素、20S蛋白酶体的变化及意义

摘要

Objective To detect the serum levels of ubiquitin (Ub) and 20S proteasome (20S PSM) in patients with chronic obstructive pulmonary disease (COPD), and their relationship with tumor necrosis factor-a(TNF-α). Methods Serum levels of Ub, 20S PSM, TNF-a were determined by enzyme-linked immunosorbent assay in 30 patients with COPD in acute exacerbation (COPD I group), 30 patients with COPD in remission (COPD Ⅱ group), and 30 healthy subjects (control group). Results As compared with control group and COPD II group, serum levels of Ub, 20S PSM, and TNF-a were significantly increased (P < 0.01) in COPD Ⅰ group. Serum levels of Ub and TNF-α were statistically increased in COPD II group as compared to control group (P < 0.01), while 20S PSM was not significantly increased. A significant correlation was found between serum levels of Ub, 20S PSM, and TNF-α. Conclusion Serum levels of Ub and 20S PSM were increased, may be related to high protein catabolism, and was associated with systemic inflammatory response in patients with COPD.%目的:检测慢性阻塞性肺疾病(COPD)患者血清泛素(Ub)、20S 蛋白酶体(20S PSM)的变化,并探讨其与肿瘤坏死因子-α(TNF-α)的相关性.方法:采用酶联免疫吸附测定法检测30 例COPD 急性加重期患者(COPDⅠ组)、30 例COPD 缓解期患者(COPDⅡ组)及30 例健康体检者(正常对照组)血清Ub、20S PSM、TNF-α水平,并进行分析.结果:与正常对照组及COPDⅡ组比较,COPDⅠ组血清Ub、20S PSM、TNF-α均显著增加(P ＜ 0.01).与正常对照组比较,COPDⅡ组血清Ub、TNF-α显著增加(P ＜ 0.01),20S PSM 增加不显著(P ＞ 0.05).COPD 患者血清Ub、20S PSM 水平分别与TNF-α具有相关性(r ＝ 0.559,r ＝ 0.859,均P ＜ 0.01).结论:Ub、20SPSM 在COPD 患者血清中含量增加,可能与COPD 患者体内蛋白质高分解代谢相关,并且其水平增加与COPD全身炎症反应因子TNF-α激活相关.