Objective To investigate the correlation between c-di-GMP and biofilm formation by Pseudomonas aeruginosa through constructing strains with phospho-diesterase gene mutation. Methods The plasmid for genetic mutation was constructed with gene engineering technique and transformed into wild Pseudomonas aeruginosa by homologous recombination. The genetic mutation strains were screened by resistance and sucrose. Results Compared with wild strains, the genetic mutation strains were more prone to form microclone and biofilm on catheter material. Conclusion The increase of c-di-GMP can promotes biofilm formation. C-di-GMP has closely correlation with nosocomial infection and drug resistance caused by Pseudomonas aeruginosa.%目的:通过构建环二鸟苷酸降解酶磷脂酶D基因突变株,探讨环二鸟苷酸与铜绿假单胞菌形成生物膜的相关性.方法:采用基因工程技术构建磷脂酶D基因突变质粒,通过同源重组转化至野生型铜绿假单胞菌PA01,抗性筛选获得基因突变株bf-.扫描电子显微镜下观察基因突变株与野生菌株生物膜表型的差异.结果:与野生菌株相比,基因突变株bf-细菌微克隆的形成增加,有利于细菌在导管材料表面形成生物膜.结论:环二鸟苷酸的增加可促进细菌生物膜的形成,与铜绿假单胞菌引起的院内感染和耐药性密切相关.
展开▼
