骨髓单个核细胞移植在脑梗死大鼠脑内分化为小胶质样细胞的实验研究

摘要

目的:探讨骨髓单个核细胞(BMMNCs)移植治疗脑梗死大鼠的疗效及其脑内分化情况.方法:采用线栓法制备大脑中动脉梗死大鼠模型,筛选成功模型后随机分为溶剂组(vehicle组)和BMMNCs移植组(BMMNCs组),BMMNCs组于24 h后经尾静脉注入1 mL Brdu标记含1×107个BMMNCs细胞悬液,vehicle组注射同等剂量PBS溶液,采用神经功能损害严重程度评分(Nss)观察神经行为学功能,免疫荧光双标法示踪BMMNCs脑内分化情况.结果:与vehicle组相比,BMMNCs组Nss明显降低(P＜0.05),BMMNCs组脑内梗死皮层区、海马齿状回检测到Brdu和Ibal双标阳性细胞.结论:BMMNCs移植在脑梗死大鼠脑内分化为小胶质样细胞,显著改善脑梗死大鼠神经行为学功能.%Objective To investigate the therapeutic effect and differentiation of bone marrow mononuclear cells (BMMNCs) transplanted in the brains of cerebral infarction rats.Methods The middle cerebral artery occlusion models (pMCAO) were established by intraluminal vascular suture method,the successful models were randomly divided into the vehicle group (vehicle group) and BMMNCs transplantation group (BMMNCs group),BMMNCs group was injected 1 mL cell suspension containing 1 × 107 BMMNCs marked by Brdu via the tail vein after 24 h,vehicle group was injected the same amount of PBS.Neurological severity scores (Nss) was used to observe neural behavioral function,double immunofluorescent labeling was utilized to trace the differentiation of BMMNCs in the rats' brains.Results Compared with vehicle group,Nss of BMMNCs group was significantly lower (P ＜ 0.05),Brdu and Ibal double-labeled positive cells were found in the infarcted cortex and hippocampal dentate gyms in BMMNCs group.Conclusion BMMNCs transplanted in the brains of cerebral infarction rats can differentiate into microglia,and improve the neural behavioral function of rats following cerebral infarction.