目的:研究SGC7901细胞与GES-1细胞中食管癌相关基因4(ECRG4)蛋白表达差异及5-杂氮-2'-脱氧胞苷(5-Aza-CdR)干预前后SGC7901细胞中ECRG4基因表达变化情况.方法:Western blot检测SGC7901细胞、GES-1细胞中ECRG4编码蛋白的表达,以不同浓度(0、5、10 μmol/L)5-Aza-CdR干预SGC7901,48 h后再以Western blot检测ECRG4编码蛋白的表达情况.结果:SGC7901细胞中ECRG4蛋白表达较低而GES-1细胞中表达较高,差异有统计学意义(P＜ 0.001).以5-Aza-CdR干预SGC7901细胞48 h,随着药物浓度增加,ECRG4蛋白表达逐渐上调,10 μmol/L处理组比5μmol/L组更明显,差异有统计学意义(P＜0.01).结论:SGC7901细胞中ECRG4蛋白的表达量明显低于GES-1细胞;以5-Aza-CdR处理SGC7901细胞48 h后ECRG4蛋白表达有上调,且10μmol/L组比5μmol/L组其恢复表达作用更明显.%Objective To study the difference of ECRG4 protein expression between SGC7901 cells and GES-1 cells and the changes of ECRG4 gene expression in SGC7901 before and after 5-Aza-CdR intervention. Methods Western blot was used to detect ECRG4 protein expression in SGC7901 and GES-1. SGC7901 cells were then intervented by 5-Aza-CdR at different concentrations (0, 5, and 10 mol/L). 48 h after intervention, Western blot was used again to determine ECRG4 protein expression. Results ECRG4 protein expressions were lower in SGC7901 and higher in GES-1, with a statistical significant difference (P < 0.001). 48 h after intervetion with 5-Aza-CdR for SGC7901, with an increase in drug concentration, ECRG4 protein expression upregulated gradually; the change was more obvious in the 10 μmol/L group than in the 5 μmol/L group, with a statistical difference (P < 0.01). Conclusions The quantity of ECRG4 protein expression is smaller in SGC7901 cells than in GES-1 cells. 48 h after treatment with 5-Aza-CdR, ECRG4 protein expression is upregulated in SGC7901, and the upregulation is more significant in the 10 μmol/L group than in the 5 μmol/L group.
展开▼
