目的:明确T140体内靶向阻断 SDF-1/CXCR4信号通路对基质细胞衍生因子-1(SDF-1)及基质金属蛋白酶(MMPs)的影响,探讨T140延缓软骨退变的作用。方法:健康9月龄雄性Hartley 豚鼠36只,随机分成A组(实验组)、B 组(实验对照组)、C 组(空白对照组),每组12只。2、4、6、8、10、12周时,ELISA法测血清中SDF-1含量。12周时,RT-PCR法测关节软骨内基质金属蛋白酶(MMP-3、MMP-9、MMP-13)mRNA的表达。结果:A组血清中SDF-1含量逐渐降低,B、C 组逐渐升高,同一时间比较,A组与B、C 组有明显差异(P <0.05);A 组MMP-3、9、13 mRNA 的表达量低于 B、C 组,差异有统计学意义(P <0.05)。结论:T140靶向阻断SDF-1/CXCR4信号通路,减少血清中SDF-1的分泌并降低软骨内MMPs mRNA的表达量,从而减缓关节软骨的退变。%Objective To explore the effect of T140 on SDF-1 and MMPs levels through targeted blocking SDF-1/CXCR4 signaling pathway , and to investigate the function of T140 to prevent from cartilage degeneration. Methods Thirty-six 9-month-old male healthy Hartley guinea pigs were divided into three groups: experimental group(group A,n = 12),experimental control group(group B,n = 12) and blank control group (group C,n = 12). In the 2nd,4th,6th,8th,10th,12th week, the levels of SDF-1 in serum were quantified with ELISA. In the 12th week, mRNA levels of MMP-3,MMP-9 and MMP-13 in articular cartilages were measured with RT-PCR. Results The serum levels of SDF-1 of the group A decreased gradually but increased in group B and C. Group A had a statistical significance compared with group B and C at the same time point (P< 0.05).The mRNA levels of MMPs in group A were lower than group B and C (P < 0.05). Conclusion T140 could block the SDF-1/CRCR4 signaling pathway and decrease the secretion of SDF-1 and mRNA expression levels of MMPs and reduce the cartilage degeneration.
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