青岛地区汉族人群脂联素基因多态性与非酒精性脂肪性肝病的相关性

摘要

Objective To investigate the association between the Adiponectin rs266729 and rs2241766 gene polymorphisms and nonalcoholic fatty liver disease in the Han Chinese population residing in Qingdao. Methods Adiponectin rs266729 and rs2241766 gene polymorphisms were genotyped in patients with NAFLD (n = 336) and healthy controls (n = 280) using polymerase chain reaction (PCR). Serum lipid profiles and adiponectin levels were determined using biochemical methods. Statistical analyses were performed using Pearson Chi square test, logistic regression analysis, t test, linear regression analysis. Results We found a significant association between the Adiponectin rs266729 genotype frequencies and allele frequencies between NAFLD pa-tients and controls (χ2= 9.929, P = 0.007; χ2= 9.809, P = 0.002). After adjustment of confounding factor, the rs266729 G allele was associated with an increased risk of NAFLD compared to the C allele (OR = 1.410, 95%CI: 1.082-1.831, P = 0.008) No significant differences were found in the rs2241766 genotype frequencies and allele frequencies between NAFLD population and the controls (OR = 1.410, 95%CI: 1.082-1.831, P = 0.008). Conclusion The Han Chinese in Qingdao carrying the rs266729 G allele are at increased risk of NAFLD.%目的：探讨青岛地区汉族人群脂联素（ADIPOQ）基因 rs266729和 rs2241766两位点的多态性与非酒精性脂肪性肝病（NAFLD）的相关性。方法：采用多聚酶链反应（PCR）及基因型检测的方法对青岛地区汉族人群338例 NAFLD 患者（ NAFLD 组）和280例正常人群（对照组）进行基因型的检测，采用 Pearsonχ2检验、Logistic 回归分析、 t 检验等方法对各组基因型、等位基因以及临床资料进行比较。结果：脂联素rs266729位点基因型及等位基因频率在 NAFLD 组与对照组之间存在明显差异（χ2＝9．929， P ＝0．007；χ2＝9．809， P ＝0．002）。校正混杂因素后，G等位基因携带者患NAFLD的风险是C等位基因的1．41倍（OR ＝1．410，95％CI：1．082～1．831，P ＝0．008）。而 rs2241766基因型及等位基因在两组中未见明显差异（χ2＝0．327， P ＝0．849；χ2＝0．074， P ＝0．785）。结论：在青岛地区汉族人群中，脂联素基因 rs266729G等位基因携带者罹患NAFLD的风险增加，而rs2241766基因多态性与NAFLD的发病无明显相关性。