目的 探讨骨髓间充质干细胞(BMSCs)移植对缺血再灌注脑组织的神经保护作用.方法实验大鼠随机分为空白组、模型组、PBS液移植组、BMSCs移植组,后三组采用改良Longa线栓法制作大鼠大脑中动脉闭塞再灌注(MCAO)模型,BMSCs移植组采用尾静脉注射法移植1 mL(即l×l06)个BMSCs, PBS液移植组尾静脉注射1 mL PBS液;分别于造模成功后12 h、1 d、3 d、7 d 4个时间点采用改良大鼠神经功能缺损评分(mNSS)评估各组神经功能缺损程度;实时荧光定量PCR技术检测各组各观测时间点miR-34a、miR-21的表达.结果(1)BMSCs移植组的mNSS评分在12 h、1 d时与模型组差异无统计学意义(P>0.05);3、7 d时明显低于模型组(P<0.05).(2)在各观测时间点,BMSCs移植组的miR-34a表达量均显著低于模型组(P<0.01),miR-21表达量均显著高于模型组(P<0.01).结论(1)BMSCs移植可明显改善脑缺血再灌注大鼠的神经功能,具有一定的神经保护作用.(2)BMSCs移植可能通过调节病灶区凋亡相关microRNA的表达发挥神经保护作用.%Objective To investigate the neuro-protective effect of bone marrow mesenchymal stem cells (BMSCs)transplantation in cerebral ischemia/reperfusion injury model. Methods The modified Zea-Longa suture method was adopted to establish rat middle cerebral artery occlusion(MCAO)/reperfusion injury model.Experimen-tal rats were randomly divided into blank group,model group,PBS group and BMSCs transplantation group. PBS group and BMSCs transplantation group were respectively received tail vein injection of 1 mL PBS or BMSCs(l × l06)6 h after the establishing of MCAO/reperfusion model. Neurological function was evaluated using the modified neurologic severity score(mNSS)scale at 12 h,1 d,3 d and 7 d after operation.Quantitative real-time PCR was used to detect the levels of miR-34a and miR-21 in ischemic brain tissue. Results(1)The mNSS scores in BM-SCs transplantation group showed no significant difference when compared with those in model group at 12 h and 1 d after operation(P > 0.05),whereas were significantly lower than those in model group at 3 d and 7 d(P <0.05).(2)Compared with model group,BMSCs transplantation group showed decreased level of miR-34a(P <0.01),while elevated level of miR-21 at different time points(P<0.01). Conclusions BMSCs transplantation can improve the impaired neurological function induced by cerebral ischemia/reperfusion injury,showing neuro-pro-tective effect.The mechanism may be associated with regulating the expressions of apoptosis-related microRNAs.
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