目的 探讨Sysmex XN血液体液分析仪体液模式对体液中高荧光细胞计数筛查肿瘤细胞的临界应用价值.方法 利用Sysmex XN血液体液分析仪体液模式测定浆膜腔积液(胸腹水和脑脊液)标本中的高荧光细胞计数(HF-BF#)和百分比(HF-BF%),同时送病理科进行液基薄层细胞学检查和免疫组化检查,以病理科报告为诊断标准,对所有数据进行统计学处理,建立ROC曲线,评估胸腹水高荧光细胞初筛恶性肿瘤的应用价值.结果 106份标本中,44例为HF-BF计数阳性,经病理科液基薄层细胞学检查和肿瘤免疫标记确认,其中有12例为恶性肿瘤.通过受试者工作特征(ROC)曲线分析阳性标本,参数HF-BF#(×106/L)、HF-BF%/100WBC的检测临界点分别为3.5和1.55.参数HF-BF#对恶性细胞检出的灵敏度(真阳性率)为66.7%,特异度(真阴性率)为40.1%,阳性预测值为27.3%,阴性预测值为91.7%,准确率为63.2%.参数HF-BF%对恶性细胞检出的灵敏度为83.3%,特异度为40.6%,阳性预测值为27.3%,阴性预测值为91.7%,准确率为63.2%.结论 Sysmex XN血液体液分析仪体液分析模式对浆膜腔积液标本进行肿瘤细胞筛查,参数HF-BF#和HF-BF%具有较高的阴性预测值,可作为恶性体液的阴性排除指标.%Objective To investigate the examination of tumor cells in pleural effusion by body fluid mod-el of Sysmex XN-9000 automated hematology analyzer ,and set the cutoff value of HF-BF# and HB-FB%for screen-ing tumor cells in pleural effusion. Methods One hundred and six pleural effusion specimens were detected by Sysmex XN-9000,and malignant samples were confirmed by TCT(Thinprep Cytology Test)and immunohistochem-istry method. Results Of 106 specimens,44 cases of HF-BF count were positive,and 12 samples were confirmed malignant by TCT and immunohistochemistry method. The cutoff value of HF-BF#(× 106/L)and HF-BF%/100WBC were 3.5 and 1.55 respectively. The detection sensitivity,specificity,positive predictive value,negative predictive value,accuracy of HF-BF# were 66.7%,40.1%,27.3%,91.7% and 63.2% respectively and those of HF-BF%were 83.3%,40.6%,27.3%,91.7%and 63.2%respectively. Conclusions HF-BF# and HF-BF%of body fluid model of Sysmex XN automated hematology analyzer are effective for screening tumor cell in pleural effusion ,and all the screening samples must be confirmed by pathology methods.
展开▼
