CD19+CD24hiCD38hi Breg细胞在胰腺癌患者外周血中的频率及其与Treg细胞间的关系

摘要

目的:探究调节性B细胞(B regulatory cells,Breg)在胰腺癌患者外周血中的表达频率及其与临床分期间的关系,并分析其与调节性T细胞(T regulatory cells,Treg)间的相关性.方法:选取明确诊断的胰腺癌患者50例及健康对照者21例,流式细胞术检测外周血CD19+CD24hiCD38hiBreg和CD4+CD25highTreg细胞表达频率,并分析这两种细胞与临床分期的关联及两者间的相关性.结果:胰腺癌患者CD19+CD24hiCD38hiBreg显著高于对照者(P＜0.05),并与临床分期密切相关;CD4+CD25highTreg同样在胰腺癌患者中异常上调,随着临床进展而逐渐增加;CD19+CD24hiCD38hiBreg和CD4+CD25high曲Treg细胞频率间呈正相关(P＜0.05).结论:CD19+CD24hiCD38hiBreg在胰腺癌患者外周血中异常上调,并与临床分期相关,提示该细胞亚群可能在胰腺癌发生发展中发挥重要作用;Breg与Treg细胞间显著正相关性提示异常表达的Breg细胞可能通过Treg细胞参与胰腺癌的致病过程.%Objectives The aim of the study was to detect the frequency of B regulatory cells (Breg)and the correlation between Breg and T regulatory cells (Treg) in pancreatic cancer patients,and to investigate its role in pathogenesis of pancreatic cancer.Methods 50 pancreatic cancer patients and 21 healthy controls were enrolled.CD19+CD24hiCD38hi Breg and CD4+CD25high Treg was also determined via flow cytometry.The correlation between Breg and Treg was analyzed by Spearman correlation test.Results Upregulation of CD19+CD24hiCD381hi Breg was associated with pancreatic cancer progression.Furthermore,this B cell subset was positively correlated with the frequency of CD4 + CD25high Treg cells.Conclusions Together,CD19 + CD24hiCD38hi Breg cells are significantly elevated in pancreatic cancer patients,indicating this B cell subset might play an vital role in clinical progression of pancreatic cancer.The significant positive correlation between Breg and Treg may suggest CD 1TCD24hiCD38hi Breg are affecting tumor progression through Treg cells.