非手术牙周治疗结合大黄素的应用对肥胖大鼠胰岛素抵抗及血清炎症因子的影响

摘要

Objective To investigate the effect of emodin on insulin resistance and serum inflammatory factors in obese rats. Methods 16 obese rats with experimental periodontitis were selected and then randomly di-vided into periodontal treatment group(control group)and periodontal therapy combined with emodin group(medi-cation group). All the rats were sacrificed 2 weeks after treatment. Before the rats were killed,the orbital vein blood was taken to detect fasting blood glucose,fasting insulin;levels of serum C-reactive protein(CRP)and tu-mor necrosis factor-α(TNF-α)were detected by enzyme linked immunosorbent assay.Results 2 weeks after peri-odontal treatment,level of CRP was significantly lower in the medication group than in the control group(t =2.170,P = 0.044);TNF-α was significantly lower in the medication group than in the control group(t = 3.074, P=0.007).Fasting blood glucose level was lower in the medication group than in the control group,and HOMA-β was higher than the control group,no statistical differences were found between the two groups.Conclusions Non-surgical periodontal treatment combined with local administration of emodin can help repair of periodontal epitheli-al barrier and reduce levels of serum inflammatory factors.%目的 探讨大黄素对肥胖大鼠胰岛素抵抗及血清炎症因子的影响.方法 选取16只已成功建立肥胖复合牙周炎模型的大鼠,随机分为牙周治疗组(对照组)及牙周治疗加大黄素组(药物组),所有大鼠于牙周治疗后2周处死.牙周治疗后2周,眼眶静脉取血,检测空腹血糖及胰岛素,运用酶联免疫吸附法检测血清C-反应蛋白(CRP)及肿瘤坏死因子-α(TNF-α).结果 牙周治疗后2周,药物组的CRP水平显著低于对照组(t=2.170,P=0.044);药物组的TNF-α显著低于对照组(t=3.074,P=0.007);药物组的空腹血糖低于对照组,HOMA-β高于对照组,两者之间差异无统计学意义.结论 牙周非手术治疗结合大黄素局部给药,有助于牙周上皮屏障的修复,可以下调血清炎症因子水平.