胡芦巴总皂苷降低非酒精性脂肪肝大鼠血脂水平及肝内脂质沉积

摘要

Objective To study the therapeutic effect of trigonella foenum greacum. L saponin(TFGs)on blood lipid and liver lipid deposition in rats with nonalcoholic fatty liver disease. Methods A rat model of high-fat diet induced nonalcoholic fatty liver disease was established. Rsts were then treated with different dosages of TFGs (low,middle and high dose)for 5 weeks. The blood lipids,liver lipid deposition,liver index (ratio of liver weight/body weight)and hepatocyte steatosis were examined. Results The serum total cholesterol (TC)levels de-creased to (2.72±0.26)mmol/L,(2.69±0.57)mmol/L and (2.12±0.38)mmol/L,respectively,and the low density lipoprotein-cholesterol(LDL-C)decreased to (1.50±0.16)mmol/L,(1.59±0.33)mmol/L and (1.15±0.24)mmol/L,respec-tively,in rats with TFGs intervention at low,middle and high doses at the end of observation,which were all sig-nificantly lower than that in the non-treated models [TC was (3.58±1.08)mmol/L,and LDL-C was (1.60±0.18) mmol/L,(P<0.01)];In liver homogenates,TGFs treatment at low,middle and high doses decreased the TC by 67.4%, 66.7% and 63.1%,respectively(P<0.01),decreased the triglyceride(TG)by 56.0%,42.6% and 52.3%,respectively(P<0.01),and decreased the liver index by 12.1%,9.1% and 6.06%,respectively (P<0.01)as compared to those in non-treated models;Significant remission of steatosis in livers was observed in high TGFs groups by histopathology. Conclusion TFGs could decrease blood lipids and ameliorates liver lipid deposition in rats with high-fat diet induced nonalcoholic fatty liver disease and maybe become a promising remedy for this disorder in human.%目的：观察胡芦巴总皂苷（TFGs）对非酒精性脂肪肝大鼠血脂水平及肝内脂质沉积的影响。方法建立大鼠高脂饮食非酒精性脂肪肝模型，给予小、中、大剂量TGFs治疗5周后，观察各组血脂、肝内脂质、肝指数和肝细胞脂肪变的变化。结果在处理后，小、中和大剂量TFGs组血清总胆固醇分别为（2.72±0.26）mmol/L、（2.69±0.57） mmol/L和（2.12±0.38）mmol/L，低密度脂蛋白分别为（1.50±0.16）mmol/L、（1.59±0.33）mmol/L和（1.15±0.24） mmol/L，较模型对照组总胆固醇为[（3.58±1.08）mmol/L，低密度胆固醇为（1.60±0.18）mmol/L均显著下降（P<0.01）]，但三种剂量处理组动物血清甘油三脂水平无显著性变化；与模型组比，小、中和大剂量TGFs组肝组织匀浆总胆固醇分别下降了67.4%、66.7%和63.1%，甘油三脂分别下降了56.0%、42.6%和52.3%（P<0.01）,肝指数分别下降了[（12.1%、9.1%和6.1%（P<0.01）]；病理学检查显示TGFs处理组动物肝细胞脂肪变程度明显降低。结论 TFGs可降低血脂水平和减少肝内脂质沉积，对高脂饮食诱发的非酒精性脂肪肝有治疗作用。