碳化二亚胺乙醇溶液表面处理对牙本质微拉伸粘接强度的影响

摘要

目的：探讨碳化二亚胺盐酸盐[1-ethyl-3-（3-dimethylaminopropyl） carbodiimide，EDC]乙醇溶液表面处理对牙本质即刻微拉伸粘接强度、断裂模式和粘接界面微观形态的影响。方法：称取不同质量EDC溶解于无水乙醇中，配制不同浓度（2、1、0．3、0．1、0．01 mol／L）的EDC乙醇溶液。将28颗人离体第三磨牙随机平分为7组：5种浓度的EDC处理组、未处理组、乙醇溶剂处理组。在低速精密切割机下去除牙合面牙釉质，暴露中层牙本质，35％（质量分数）磷酸凝胶酸蚀后根据不同分组对脱矿牙本质表面分别进行处理60 s，使用全酸蚀粘接剂（Single Bond 2）粘接处理后，堆塑复合树脂并固化。室温下保存24 h后，制备标准条形试件并测定微拉伸粘接强度，在体视显微镜下对试件的断裂模式进行观察。结果：2 mol／L组[（22．17±13．31） MPa]和1 mol／L组[（45．31±17．80） MPa]的粘接强度显著下降（P＜0．05），粘接断裂模式发生的比例明显提高，分别为81．2％和41．3％。其余各组粘接强度差异无统计学意义（P＞0．05）。结论：0．3、0．1和0．01 mol／L的EDC乙醇溶液表面处理对牙本质即刻粘接强度无明显影响，2 mol／L和1 mol／L的EDC乙醇溶液表面处理会显著降低牙本质即刻粘接强度。%Objective:To evaluate the microtensile bond strength changes and patterns of fractures of the bonding interface after dentine surface treatment with carbodiimide-ethanol solution .Methods:1-eth-yl-3-(3-dimethylaminopropyl) carbodiimide (EDC) dissolved in ethanol was diluted into different con-centrations of 2, 1, 0.3, 0.1 and 0.01 mol/L EDC-ethanol solutions.Twenty-eight caries-free extracted human third molars were ground metallurgically to prepare flat occlusal mid-coronal dentin surfaces and etched with 35%( mass fraction) phosphoric acid gel .Then they were treated with EDC-ethanol solution for 60 s before the bonding procedure and randomly divided into five experimental groups corresponding to the tested EDC-ethanol concentrations .The ethanol treated and no pre-treated surfaces were used as con-trols.Single Bond 2 adhesive was applied and resin composite disk was stacked on the treated dentine surface .The teeth with resin composite disks were stored in water at room temperature for 24 h and then sectioned longitudinally to produce stick specimens for microtensile bond strength test .Fracture patterns were observed with a stereomicroscope .Results:The dentin surfaces pre-treated with 2 mol/L [ (22.17 ± 13.31) MPa] and 1 mol/L [(45.31 ±17.80) MPa] EDC-ethanol solutions resulted in statistically sig-nificant lower bond strength value ( P<0 .05 ) .Increasing numbers of fracture pattern at the resin-dentin interface were also found in this two groups with percentages of 81 .2%and 41 .3%respectively .No sig-nificant difference was observed in the groups with 0.3, 0.1, 0.01 mol/L EDC surface treatment ( P>0.05).Conclusion:No significant difference of immediate bond strengths was found in the 0.3, 0.1, 0 .01 mol/L groups compared with the control group .EDC-ethanol solution surface treatment with con-centrations of 2 mol/L and 1 mol/L resulted in decreasing of the bonding strength .