花生单粒种子脂肪酸含量的气谱快速无损测定

摘要

A protocol for analyzing 8 fatty acids using 5-20 mg groundnut seed tissue was described, which required 35 min for preparation at room temperature and 18 min for gas chromatography analy- sis. The results of analysis proved to be accurate and reproducible, irrespective of sampling amount and fatty acid content. The protocol caters the need for non-destructive sampling and gene pyramiding in genetic and breeding studies in groundnut, and may facilitate genetic improvement in fatty acid composition of the groundnut crop.%描述了一种利用5～20mg花生种子组织测定花生8种脂肪酸的方法。于室温下制备用于色谱分析的样品耗时35min,气相色谱分析则需要18min。研究结果证实,本法准确性高,重复性较好,可满足花生遗传育种研究中对种子无损取样及基因聚合育种需要,将为花生脂肪酸成分的遗传改良提供便利。