Objective:Myriad scaffolding materials and compositions have been and are still being tested for the regeneration of each very specific hard or soft tissue. The objective of this study was to examine a novel composite poly (lactic-co-glycolic acid) / poly (e-caprolactone) / nano-carbonate hydroxyapatite (PLGA/PCL/nHA) on the initial behavior of bone marrow stromal cells (BMSCs) and evaluate the proliferation and differentiation of BMSCs grown on composite scaffold in vitro. Methods: BMSCs cells were cultured on PLGA/PCL/nHA in vitro, cell viability was assessed by MTT reduction assay. Cell adhesion on PLGA/PCL/nHA was observed by scanning electron microscopy (SEM). Results: BMSCs cells viability were not affected, the proliferation and growth were not affected by PLGA/PCL/nHA, and BMSCs cells could be attached to with a spindle shape or a polygonal shape and extended the pseudopod on PLGA/PCL/nHA. Conclusions: The composite scaffolds is biocompatible and crucial in regulating cellular functions, including survival, adhesion, proliferation, and the like.%目的:研究新型复合支架聚乳酸-聚乙醇酸/聚己内酯/纳米羟基磷灰石(PLGA/PCL/nHA)的生物相容性,探讨其作为细胞培养材料和骨组织工程支架的可行性.方法:将兔骨髓基质细胞(bone marrow stromal cells,BMSCs)接种于PLGA/PCL/nHA复合支架上,体外共同培养后,MTT法检测BMSCs增殖活性,电镜下观察PLGA/PCL/nHA复合支架材料表面和孔隙内细胞附着情况.结果:BMSCs在PLGA/PCL/nHA复合支架材料上生长良好.PLGA/PCL/nHA材料表面和孔隙内均有细胞附着,细胞已伸展成梭形或多角形,伸出伪足黏附于材料上.结论:新型PLGA/PCL/nHA复合支架材料生物相容性好,是一种良好的组织工程支架材料,可作为种子细胞的载体应用于骨组织工程中.
展开▼
