激素对小麦幼胚胚性无性系高频率诱导的影响

摘要

研究了3种外源激素对3个小麦品种(系)幼胚体细胞胚性无性系高频率形成的影响。结果表明：2,4-D能显著促进小麦幼胚体细胞胚性无性系的形成，并存在着基因型间的差异，在小麦体细胞无性系形成中起主要作用。其最佳适用质量浓度为2.0～3.0 mg/L，2,4-D的这种作用能被培养基中加入的细胞分裂素所加强，这种作用在不同的细胞分裂素间是有差异的，在质量浓度0.1～2.0 mg*L-1内，随KT浓度的升高，6-BA浓度的降低，胚性愈伤诱导率提高。%Effect of three exogenous hormones on high frequency induction of three varieties of wheat (Triticum aestivum L.) immature embryos is studied.The following results are acquired.Concentration of 2,4-D in media significantly affects the induction of embryogenic calli derived from immature embryos of wheat,with diffe rent frequency between the three genotypes.The most appropriate concentration of 2,4-D is at 2.0 ～3.0 mg/L.This capability of 2,4-D can be enforced by the ad dition of KT or 6-BA in medium.But this enforcement differs between KT and 6-B A.KT has a significant role in the induction of embryogenic calli at relatively high concentration while 6-BA works well at lower concentration.