烈香杜鹃嫩茎腋芽丛生芽诱导和植株再生

摘要

【目的】建立烈香杜鹃嫩茎段腋芽丛生芽诱导和植株再生体系,为烈香杜鹃工厂化育苗奠定技术基础。【方法】以烈香杜鹃嫩茎为外植体,应用均匀设计法对影响烈香杜鹃腋芽丛生芽诱导和生根的各主要植物生长调节剂及其水平的作用进行探讨。【结果】适宜的烈香杜鹃嫩茎段腋芽丛生芽诱导培养基为：1/2DR＋TDZ 2.80mg/L＋IAA 0.01mg/L＋GA31.85mg/L,诱导率为99.8%;生根培养基为：1/2DR＋IAA 0.07mg/L＋NAA 0.02mg/L,生根率达99.7%。在生根培养基中添加GA32.00mg/L,再生植株茎节在35d的培养周期内,每段增殖倍数平均达5以上,植株再生率为97.0%。再生植株炼苗移培后成活率达95.0%以上。【结论】建立了烈香杜鹃嫩茎段腋芽丛生芽植株再生体系。%【Objective】 The study was to establish the axillary multiple shoots induction from the young stems and plantlet regeneration system of Rhododendron anthopogonoides,which could establish a technological foundation for factory seedlings of R.anthopogonoides.【Method】 The young stems of R.anthopogonoides were used as explants,and the main plant growth regulators with different levels of the axillary multiple shoots induction from the young stems and shoots rooting were investigated through uniform design experiments.【Result】 The results showed that 1/2 DR+TDZ 2.80 mg/L+IAA 0.01 mg/L+GA3 1.85 mg/L was fit for the axillary multiple shoots induction,the rate of induction was 99.8%;1/2 DR+IAA 0.07 mg/L+NAA 0.02 mg/L for rooting,the rate of rooting was 99.7%.The gibberellin(GA3)2.00 mg/L was added in the rooting medium.Stems of regenerated shoots were cultured,and a 5-fold proliferation rate was achieved within 35 days,the rate of plantlet regeneration was 97.0%.The survival rate of regenerated plants was more than 95.0%.【Conclusion】 The axillary multiple shoots induction from the young stems and plantlet regeneration system of R.anthopogonoides have been successfully established.