SSR和SCoT标记在美洲黑杨×青杨派杂种无性系遗传差异性分析上的比较

摘要

[Objective] This study aimed to compare the applicability of genetic diversity of SSR and SCoT markers in Populus deltoides × Section tacamahaca hybrids.[Method] SSR and SCoT markers were used to analyzed genetic difference of 9 P.deltoides × S.tacamahaca hybrids and 3 parents.[Result] A total of 94 bands were produced by selected 12 SSR primer pairs,in which polymorphic bands were 85 (accounting for 90％) and the marker index was 3.19.The selected 14 SCoT primers produced 127 bands,in which polymorphic bands were 95 (accounting for 75％) and the marker index was 2.72.Cluster analysis showed that the genetic similarity coefficients of 12 clones by SSR and SCoT markers were 0.44-0.80 with average of 0.62 and 0.40-0.87 with average of 0.64,respectively.The 12 poplar materials were divided into three and five categories by SSR and SCoT markers based on the averages of genetic similarity coefficients.Mantel test revealed that the relation of genetic similarity matrix between the two markers was significant (r=0.501 3,P=0.003) for the 12 clones.[Conclusion] Both markers were suitable for identification and genetic diversity of P.deltoides × S.tacamahaca hybrids,and SSR markers had higher polymorphic bands ratio and marker index than SCoT markers.%[目的]比较SSR和SCoT分子标记在美洲黑杨×青杨派杂种无性系遗传差异性分析上的应用性.[方法]利用SSR和SCoT标记,对9个美洲黑杨×青杨派杂种无性系及3个亲本的遗传差异性进行分析.[结果]筛选出的12对SSR引物对供试杨树材料共扩增出94条清晰条带,其中多态性条带85条,多态性比率达到90％,标记指数为3.19;筛选出的14条SCoT引物对供试杨树材料共扩增出清晰条带127条,其中多态性条带95条,多态性比率达到75％,标记指数为2.72.聚类分析表明,SSR和SCoT分子标记得出12个无性系的遗传相似系数分别为0.44～0.80和0.40～0.87,平均遗传相似系数分别为0.62和0.64;在相似系数平均值处,SSR和SCoT分子标记将12个杨树材料分别分为3大类和5大类.Mantel检测显示,2种分子标记在12个无性系间的遗传相似性显著相关(r=0.501 3,P=0.003).[结论]这2种分子标记均适合美洲黑杨×青杨派杂种无性系鉴定和遗传多样性研究,但SSR标记的多态性比率和标记效率均高于SCoT标记.