白桦木材蓝变生防菌(Bacillussubtilis)B26液体发酵条件的优化

摘要

The fermentation culture medium for the strain B26 was studied by single factor experiments and orthogonal experi ments. The optimal culture medium formula was determined as sucrose 1.5% , peptone 1.5% , CaCl2 0. 15% , and Mg-S04 ? 7H2O 0. 20% . The fermentation conditions were obtained by single factor experiments as temperature 35 degrees C, initial pH of the medium 7.5 , fermentation fluid 120 mL in a 250 mL conical flask, inoculation volume 8% , rotation speed 130 r/min, and fermentation time 72 h. The inhibition zone diameter of the fermentation liquid reached 19. 00 mm under this optimized conditions.%采用单因素试验法和正交试验法对生防菌株B26液体发酵条件进行了优化,得到最适发酵培养基配方为:蔗糖1.5％、蛋白胨1.5％、CaCl2 0.15％、MgSO4·7H2O 0.20％；采用单因素试验法对其培养条件进行优化,确定最适培养条件为:温度35℃、初始pH值7.5、锥形瓶体积为250 mL,发酵液为120 mL,接种量8％、转速130 r/min,发酵时间72 h.在此条件下,B26菌株发酵液对白桦木材蓝变茵的抑菌圈直径可达19.00 mm,抑菌活性比优化前提高了53％.