萱草新品种组培再生体系的建立

摘要

An experiment was conducted to establish a tissue culture regeneration system of Hemerocallis ‘ Fooled Me’ by explant selection, callus induction, auxiliary bud multiplication, rooting and transplanting. Results indicate that the way for regeneration of Hemerocallis ‘ Fooled Me’ is organogenesis. Explant type and 6-BA concentration had significant effects on callus induction. The optimal explant was the branching stem of young scape and the receptacle of bud whose diameter was less than one centimeter, which were first disinfected with 75％ alcohol for 30 s, then immerged in 0.5％ NaClO for 14 min. The best medium for initiation was MS supplemented with 2.0 mg/L 6-BA and 0. 0l mg/L NAA. The multiplication of clusters was influenced by ratio of 6-BA to NAA and the best ratio was 50. The optimum medium for proliferation of Hemerocallis ‘ Fooled Me’ was MS with 0.5 mg/L 6-BA and 0. 00 mg/L NAA; and the best medium for rooting was 1/2 MS with IBA 0.5 mg/L. The survival rate of Hemerocallis ‘ Fooled Me’ was the highest on the medium for transplanting with volume ratio of vermiculite to perlite of 1: 1.%为建立萱草新品种(Hemerocallis 'Fooled Me')组培再生体系,对其外植体筛选、愈伤组织诱导、丛生芽增殖、生根、移栽等进行了试验.结果表明:Hemerocallis 'Fooled Me'的再生方式为器官发生型.外植体种类及6-BA质量浓度对愈伤组织的诱导有显著的影响,最佳外植体为幼嫩花茎分枝处茎段和＜1 cm花蕾上的花托;外植体的最佳灭菌方法为75%C2H5OH(30 s)+0.5%NaClO(14 min);最佳启动培养基为MS+6-BA2.0mg/L+NAA0.01ng/L.6-BA/NAA影响丛生芽的增殖,以50倍最好,其适宜的增殖培养基为MS+6-BA0.5 mg/L+NAA0.01 mg/L.Hemerocallis 'Fooled Me'试管苗最佳生根培养基为1/2MS+IBA0.5 mg/L.移栽基质中以蛭石和V(蛭石):V(珍珠岩)=1:1的成活率较高.