矮壮素对萱草组培苗生根及移栽的影响

摘要

With 1/2MS culture medium containing different content and hormone combinations of NAA and CCC for the rooting culture, we studied the regenerate roots and the transplanting on Hemerocallis ‘Nameless Pink’.Within a certain range, the hormone of NAA has a better effect on overall rooting rate than CCC while using NAA and CCC alone , separately .Un-der hormone of NAA culture , it is easy to form a large number of callus and roots with slender , fragile, and easily broken features;however , under hormone of CCC culture , it is easy to form quantity roots with stubby toughness and without cal-lus formation, but it can make seedling growth suppress lead to a lower root rate .For Hemerocallis ‘Nameless Pink’, it can significantly improve rooting rate and increase the quality of seedlings when combined with the 0.4 mg/L NAA and 0.5 mg/L CCC, and the transplant survival rate can be as high as 100%.%以1/2MS为基本培养基，添加不同质量浓度萘乙酸（NAA）和矮壮素（CCC），研究其对萱草新品种‘无名粉’组培苗生根及移栽的影响。结果表明：在一定范围内，单独使用NAA和CCC时，NAA的整体生根率好于CCC的整体生根率，但单独使用NAA生根的过程中发现，组培苗基部与根连接处易形成大量的愈伤组织，并且根细长脆弱、较易断；而单独使用CCC生根的过程中未见大量的愈伤组织形成，并且生成的根数量多、粗短、韧性强，但其却抑制组培苗的生长，生根率相对较低。对萱草新品种‘无名粉’来说，当配合使用0．4 mg・ L-1 NAA和0．5 mg・ L-1 CCC时，能显著提高生根率，提高苗的质量，生根试管苗移栽成活率可高达100％。