钙离子载体诱发马鹿精子顶体反应效果1）

摘要

Wapiti (Cervus elaphus) sperm was capacitated in vitro by swimming-up and the acrosome reaction (AR) was in-duced by Calcium ionophore A23187 to choose an optimal A23187 system during AR.A23187 of 0.01-100 μmol/L can enhance the ratio of AR on the capacitated sperm and 0.1μmol/L A23187 can obtain the highest ratio of AR (74.00%± 2.62%) for 5-min treatment time.AR of capacitated sperm can, but the uncapacitated sperm cannot, be induced by A23187 in wapiti.%以马鹿（ Cervus elaphus）为试验动物，采用上浮法诱导马鹿精子体外获能，以钙离子载体（ A23187）诱导马鹿精子顶体反应，进而摸索钙离子载体（ A23187）诱发其顶体反应的适宜体系。结果表明：0．01～100．00μmol/L的A23187可提高获能马鹿精子的顶体反应率；其中适宜的A23187浓度为0．10μmol/L，反应的时间为5 min，顶体反应率为（74．00±2．62）％。 A23187能促进获能马鹿精子的顶体反应，但不能显著（P＞0．05）促进未获能马鹿精子的顶体反应。