红刺玫挥发物成分及抑菌作用

摘要

利用固相微萃取与GC－MS系统，分析了红刺玫花、叶的自然挥发性物质组成。鉴定出叶片挥发物成分10种，主要成分乙酸顺式－3－己烯酯（41．84％）、1R－α－蒎烯（27．11％）、罗勒烯（6．21％），占挥发物总质量分数的75．16％；花瓣挥发物成分25种，主要成分 D－香茅醇（41．9％）、苯乙醇（15．62％）、1R－α－蒎烯（13．4％），占挥发物总质量分数的70．92％。利用β－蒎烯、罗勒烯、D－香茅醇、3－蒈烯4种挥发性物质进行了抑菌活性试验，发现4种挥发物质对供试菌均表现出一定的抑制作用，其中罗勒烯与D－香茅醇对金黄色葡萄球菌抑制作用较强，最低抑菌质量浓度（ MIC）均为12．5 mg· L－1；β－蒎烯与D－香茅醇对枯草芽孢杆菌、D－香茅醇对大肠杆菌抑制作用次之，MIC均为50 mg· L－1。β－蒎烯对大肠杆菌、金黄色葡萄球菌抑制作用较弱，MIC为800 mg· L－1。%We studied the chemical composition of the volatile organic compounds ( VOCs) from Rosa multiflora var.Cathayensis by SPME-GC-MS.Ten compounds were identified in VOCs from leaves , mainly including 41.84%( Z)-3-Hexen-1-ol ace-tate, 27.11%1Ra-lpha -Pinene and 6.21%Ocimene, and the volatile of these major constituents were 75.16%.Twenty-five compounds were identified in VOCs from the flower , mainly including 41.9%D-Citronellol, 15.62%Phenylethyl Alcohol and 13.4%1R-alpha-Pinene, accounting for more than 70%.Through antibacterial experiment, beta-Pinene, Ocimene, D-Citronellol and 3-Carene had antimicrobial activity on testing bacteria .The bacteriostasis of Ocimene and D-Citronellol on Staphylococcus aureus were most powerful, the minimal inhibitory concentrations (MIC) were both of 12.5 mg· L-1.The antibacterial effect of beta-Pinene and D-Citronellol on Bacillus subtilis, D-Citronellol on Escherichia coli were second.All of the MIC were 50 mg· L-1 .The bacteria inhibiting effect on Escherichia coli, Staphylococcus aureus of beta-Pinene was weak with the MIC of 800 mg· L-1 .