阿特拉津和毒死蜱对鲤胚胎发育的影响

摘要

研究旨在探讨不同浓度阿特拉津（Atrazine， ATR）、毒死蜱（Chlorpyrifos， CPF）及其混合物对鲤（Cyprinus carpio L.）胚胎发育、孵化率及抗氧化能力的影响。将鲤受精卵分别暴露在各自浓度为12.5、25、50、100、200μg·L-1的ATR、CPF及ATR-CPF 1��1混合液中，经96 h试验，观察发育形态，统计死亡率和畸形率，检测全鱼抗氧化能力。在暴露试验中，各处理组死亡率和畸形率随药物浓度增长呈上升趋势；初孵仔鱼体内SOD、GPx和CAT活性整体呈下降趋势。结果表明，阿特拉津和毒死蜱对鲤受精卵具有一定的致死、致畸效果，且两者生物毒性具有一定的叠加效应和协同作用，氧化胁迫是阿特拉津和毒死蜱对鲤发挥毒性作用的重要机制之一。%This study was aimed to explore the influence of different concentrations of atrazine (ATR), chlorpyrifos (CPF) and a mixture of them on embryonic development, hatchability and antioxidant capacity of common carp (Cyprinus carpio L.). The carp fertilized eggs was exposed to the concentrations of 12.5, 25, 50, 100, 200μg·L-1 ATR, CPF and ATR-CPF 1��1 mixture, respectively. After 96 h exposure, we observed their development of shape, and counted the death rate and aberration rate of the zygotes, detected antioxidant capacity of whole fish. In the exposure test, Mortality and malformation rate of treatment groups grow along with the drug concentration was on the rise;SOD, GPx and CAT activities in the newly hatched larvae of common carp as a whole were on the decline. The results indicated that ATR and CPF had some lethal and teratogenic effect on zygote of common carp, moreover, to a certain degree extent, the biotoxicity of these two toxic substances had additive effect and synergy, oxidative stress was one of the pivotal mechanism which ATR and CPF brought toxic effect to common carp.