Objective:To establish a method for determination of seven kinds of HPLC Weitong capsule of piperine content, in order to effectively control the preparation of piperine quality. Methods:the Inertsit C18 column (4.6mm × 250mm,5um) is a chromatographic column;methanol-water(77∶23)as mobile;detection wavelength:343nm;column temperature30 degrees Celsius;velocity 1.0ml·min-1. Results:Pepper alkali amount of sample in the 4.275~85.52μg·ml-1 within a good linear relationship(r=0.9998),the average recovery was 98.4%,RSD was 0.6%(n=5),the sample in the 24h stability.Conclusion: the established method of determination the content of simple and rapid, accurate and reliable results, can be used for the quality control of seven flavor capsule for treating stomachache.% 目的：建立HPLC法测定七味胃痛胶囊中胡椒碱的含量,以有效控制该制剂胡椒碱的质量.方法：以Inertsit C18柱(4.6mm×250mm,5um)为色谱柱；以甲醇-水(77∶23)为流动相；检测波长343nm；柱温30℃；流速1.0ml·min-1.结果：胡椒碱进样量在4.275~85.52μg·ml-1范围内线性关系良好(r=0.9998),平均回收率为98.4%,RSD为0.6%(n=5),样品在24h内稳定.结论：本文建立的含量测定方法简捷快速,结果准确可靠,可用于七味胃痛胶囊的质量控制.
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