HPLC法测定苦豆草药材中生物碱的含量

摘要

Objective Establish a method Sophora medicinal alkaloid content. Methods by HPLC column was Agilent Zorbax SB-C 18 column (250mm ×4.6mm,5μm);mobile phase of acetonitrile-0.05mol·L-1 potassium dihydrogen phosphate (triethylamine 2.0ml/L), gradient elution off;flow 1.0ml/min, column temperature was 30℃;detection wavelength of 205nm. Results The main alkaloid sophoridine a good linear relationship ·ml-1~0.2412mg·ml-1 range of 0.00804mg, recovery was 99.3%, RSD was 1.63%; Oxymatrine at 0.00808mg· ml-1~0.2424mg·ml-1 has a good linear relationship within the range of recovery was 99.4%, RSD was 1.82%. Conclusion The method is simple, accurate, reproducible and can be used for quantitative analysis of alkaloid methods.%目的：建立苦豆草药材中生物碱含量的测定方法。方法：采用HPLC法测定，色谱柱为Agilent TC-C18（2）（250mm×4.6mm，5μm）；流动相为乙腈-0.05mol·L-1磷酸二氢钾水溶液（三乙胺2.0ml/L），梯度洗脱；流速为1.0ml/min，柱温30℃；检测波长205nm。结果：苦豆草中主要生物碱槐定碱在0.00804mg·ml-1～0.2412mg·ml-1范围内有良好的线性关系，回收率为99.3%，RSD为1.63%；氧化苦参碱在0.00808mg·ml-1～0.2424mg·ml-1范围内有良好的线性关系，回收率为99.4%，RSD为1.82%。结论：该方法简便易行、结果准确、可靠，重复性好，可用于苦豆草中生物碱的定量分析方法。