小鼠急性酒精性肝损伤模型的制备及观察

摘要

Objective:To establish an alcohol-induced acute liver injury model in mice and to dynamically monitor the changes of biochemical indexes in serum and liver. Methods :Eighty ICR mice were randomly divided into eight groups, four control groups and four model groups. The model groups were given 52%(V/V)alcohol with 12ml·kg-1d-1 by gastric perfusion while the control groups were given distilled water. At 2, 4, 6, 8 days after administration, a model group and a control group were respectively sacrificed to take serum samples for measurements of ALT and TG levels, and liver samples for measurements of liver index, TG, SOD and MDA levels. Results:After treating for 6 days, serum ALT and TG levels were significantly increased(P<0.01), and liver index, TG and MDA level in liver tissue were increased significantly(P<0.01), whereas SOD level in liver tissue decreased significantly(P<0.01). Conclusion :A mice model of alcohol-induced acute liver injury has been successfully established, which is stable and can be used for studying the mechanism of drug protection in alcoholic liver disease.%目的：建立小鼠酒精性肝损伤模型，并动态研究小鼠血清和肝脏的生化指标的变化。方法：将80只清洁级ICR小鼠随机分为8个组：4个模型组和4个空白组。模型组小鼠按12ml·kg-1d-1胃灌注52度白酒，空白组小鼠给予胃灌注等量蒸馏水，分别在灌胃后2d，4d，6d，8d各取一组模型组和空白组小鼠，测定血清ALT、TG，肝脏指数，肝组织SOD和MDA。结果：与同时段空白组比较，造模6d后，小鼠血清中ALT和TG均显著升高（P<0.01），肝脏指数、肝组织中TG和MDA显著升高（P<0.01），而SOD活性显著下降（P<0.01）。结论：本实验在短时间内成功建立小鼠急性酒精性肝损伤模型，稳定性好，可应用于护肝药物保护机制的研究。