姜黄素脂质体的制备及包封率测定

摘要

目的：探讨姜黄素脂质体的制备，建立其含量测定方法，测定其包封率。方法：采用薄膜分散法制备姜黄素脂质体，采用超速离心法分离脂质体及游离药物，采用紫外分光光度法（UV）测定姜黄素含量，计算其包封率。结果：采用薄膜分散法制得的姜黄素脂质体为黄色，呈圆形或类圆形，平均粒径为0.49μm，分布均匀，脂质体的平均包封率为96.02%，符合2010版《中国药典》的要求。姜黄素脂质体中姜黄素含量为9.87mg/g，RSD为0.99%。结论：薄膜分散法可以简单快速地制备姜黄素脂质体，操作流程掌握容易，制备的脂质体形态较好、包封率较高；超速离心法与紫外分光光度法结合可以准确、可靠地测定姜黄素脂质体的含量。%Objective:TO discuss how to prepare the liposome of Sculellaria barbata flavones,establish the method of total flavonoids determination, and determate its encapsulation rate.Method:By using the film dispersion method to prepare the liposome of Sculellaria barbata flavones,and by using the ultracentrifugation to liposome and free drugs, and by using ultraviolet spectrophotometry (UV) to determinate the total content of flavonoids, and determate its encapsulation rate.Result:The Liposome color is dark brown,round or class round,the average particle size was 0.54 microns, uniform distribution,and the average of liposome encapsulation rate is 94.38%, it is in line with the requirements of "Chinese pharmacopoeia" 2010 version.Conclusion:The film dispersion method is easy and simple to preparate the Sculellaria barbata flavones Liposome,the operation is easy to grasp,the morphology of liposomes prepared well and high encapsulation rate;The Ultracentrifugation method combined with UV can be accurate, reliable to determinate of the encapsulation rate.