Objective To investigate the effect of Lycium Barbarum Polysaccharide combined with paclitaxel on the proliferation,cell cycle and apoptosis of HO-8910PM cell line and to explore its possible mechanism. MethodsThe human epithelial ovarian cancer HO-8910PM cells in logarithmic growth phase were divided into four groups: A: negative control group;B: positive control group paclitaxel(PTX)monotherapy(0.001,0.005, 0.01. 0.05,0.5,0.1,1μg·mL-1);C: Lycium Barbarum Polysaccharides(LBP)group(50,100,150,200,250,300, 350,400μg·mL-1);D: LBP and PTX synergy group(LBP+PTX:50+0.01,100+0.01,50+0.05,100+0.05μg·mL-1). 48 hours after drug intervention,cell proliferation inhibition rate was detected by CCK8 while apoptotic morphological changes of HO-8910PM cells were observed by Hochest staining. Effect on cell cycle and apoptosis analysis were detected by flow cytometry after drug intervention.ResultsCompared with the control group,LBP and PTX single drug effect of visible human ovarian cancer HO-8910PM cell proliferation inhibition,inhibition enhanced with time and dose increasing. LBP 48h IC50 was 280μg·mL-1,the minimum effective concentration was 120μg·mL-1 and 85% cell inhibition could be achieved at 400μg·mL-1. PTX 48h IC50 was 0.005μg·mL-1 and 100% cell inhibition could be achieved at 0.5μg·mL-1. The synergistic inhibition of LBP with PTX was greater than the sum of single agent,with significant gain effect(P<0.05). Flow cytometry analysis showed that under the effect of LBP combined with PTX,the proportion of cells in G0/GI and G2/M phases increased,apoptosis rate increased and the effects were greater than single drug effect. Synergistic effects on cell cycle inhibition and apoptosis induction were significant(P<0.05).ConclusionLBP can inhibit the proliferation and promote the apoptosis of human epithelial ovarian HO-8910PM cells. LBP can enhance significantly the proliferation inhibition and apoptosis inducing effect of paclitaxel on HO-8910PM cells.%目的 研究枸杞多糖对紫杉醇诱导的人上皮性卵巢癌HO8910PM细胞株的增殖及细胞周期、细胞凋亡的影响,并探讨其可能的作用机制.方法 将处于对数生长期人上皮性卵巢癌HO-8910PM细胞分4组:A:阴性对照组;B:阳性对照组紫杉醇(PTX)单药作用组(0.001、0.005、0.01、0.05、0.1、0.5、1μg·mL-1);C:枸杞多糖(LBP)作用组(50、100、150、200、250、300、350、400μg·mL-1);D:LBP及PTX协同作用组(LBP+PTX:50+0.01、100+0.01、50+0.05、100+0.05μg·mL-1).药物干预48h后,采用CCK8检测药物干预后细胞增殖的抑制率;Hochest染色观察HO-8910PM细胞的凋亡形态变化;流式细胞术分析药物作用后对细胞周期、凋亡的影响.结果 与阴性对照组比较,LBP、PTX单药作用可见人上皮性卵巢癌HO-8910PM细胞增殖抑制,抑制作用随时间、剂量的增强而增加,LBP作用48h的IC50为280μg·mL-1,最低起效浓度为120μg·mL-1,在400μg·mL-1时可达到85%细胞抑制,PTX作用48h的IC50为0.005μg·mL-1,在0.5μg·mL-1时达到100%细胞抑制.LBP协同PTX作用细胞可见增殖抑制大于单药作用之和,增益作用显著(P<0.05);流式细胞分析结果显示LBP协同PTX作用可致G0/GI期、G2/M期细胞比例增加,细胞凋亡率升高,效果均大于单药作用之和,协同作用对细胞周期抑制、 诱导凋亡作用的增益作用显著(P均<0.05).结论 枸杞多糖具有抑制人上皮性卵巢癌HO-8910PM细胞增殖并促进其凋亡的作用,且枸杞多糖明显增益紫杉醇对HO-8910PM增殖抑制及诱导凋亡作用.
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