鲭鱼罐头中7种多氯联苯的测定--亚临界水萃取-气相色谱法

摘要

A method for the simultaneous determination of 7 polychlorinated biophenyls (PCBs) in canned Mackerel by sub-critical water extraction coupled with gas chromatography is developed. PCBs are extracted by 200 ℃ sub-critical water for 40 min, and adsorbed on active carbon fibers at the same time. And then, PCBs are desorbed by 2 mL ethyl acetate. The results show that the linear range for the determination of PCBs in canned Mackerel is between 0.001~0.100 μg/mL with the limit of detection (LOD, S/N=3) of 0.016~0.046 μg/kg and the limit of quantification (LOQ, S/N=10) of 0.048~0.139 μg/kg. At the spiked level of 2.0、10.0、50.0 μg/kg (n=6), the recoveries range from 79.4% to 91.0% with the relative standard deviations (RSDs) between 3.1% and 12.3%. The developed method is environmentally-friendly and suitable for the simultaneous determination of 7 PCBs in canned Mackerel with high accuracy and high precision.%建立了同时检测鲭鱼罐头中7种多氯联苯（PCBs）的亚临界水萃取-气相色谱分析方法。亚临界水中加入活性炭纤维为固相萃取剂，在200℃的条件下，提取40 min，随后，用2 mL乙酸乙酯洗脱。结果表明，该方法测定PCBs含量的线性范围为0.001～0.100μg/mL；在2.0、10.0、50.0μg/kg （n=6）的添加水平下，回收率在79.4%~91.0%之间，测定结果的相对标准偏差（RSD）为3.1%～12.3%；检出限（S/N=3）为0.016~0.046μg/kg；定量限（S/N=10）为0.048~0.139μg/kg。该方法绿色环保、稳定、可靠，适用于鲭鱼罐头中多氯联苯的检测。