慢性乙型肝炎患者乙型肝炎病毒C基因启动子变异的初步研究

摘要

为建立快速特异检测乙型肝炎患者血清乙型肝炎病毒(HBV)DNA的聚合酶链式反应(PCR)方法，并初步分析患者体内乙型肝炎病毒C基因启动子(HBV.CP)变异的情况，对PCR法扩增的HBVDNA直接测序，并应用计算机进行DNA同源性分析。结果显示：应用PCR法扩增20份慢性乙肝患者血清阳性率为95%(19/20)；选择中度、重度乙肝患者血清和pGEM.7Z-HBV质粒扩增的HBV.CP各1份分别测序，与报告基因的同源性分别为72.0%、66.5%、90.0%。研究表明，PCR法可用于HBV.CP区变异的检测，慢性乙型肝炎患者存在该区变异。%To establish PCR for detection hepatitis B virus (HBV) DNA andDetect hepatitis B virus core promoter (CP) mutations in patients with hepalitis B,polymerase chain reaction amplified HBV DNA fragments were directly sequenced and common-source analyzed with the help of computer. The results showed that the positive rate of serum samples from 20 HB patients was 95%(19/20).The HBV CP fragments of two patients and pGEM.7 Z-HBV plasmid were sequenced. Compared with report gene sequence,the identical rate of 2 patients were only 72.0%,66.5% respectively,but pGEM. 7 Z-HBV plasmid was 90.0%.This study indicated that PCR may be used in the detection of HBV with mutations in CP region and CP region mutation exists in patient with chronic hepatitis B.