白花蛇舌草总黄酮对鼻咽癌细胞株CNE1增殖和凋亡的影响

摘要

目的:探讨白花蛇舌草总黄酮(FOD)对鼻咽癌细胞株CNE1的增殖和凋亡的影响.方法:体外培养鼻咽癌细胞株CNE1,使用不同质量浓度的FOD作用24、48 h,分别使用CCK-8测定法检测细胞存活率,Annexin V-FITC凋亡检测试剂盒检测细胞凋亡的情况.结果:FOD对鼻咽癌细胞株CNE1具有抑制增殖作用,24h的半数抑制量(IC50)为(27.0±2.5)mg/L,48 h的IC50为(12.1±1.3) mg/L,在一定质量浓度范围内,呈剂量-效应关系和时间-效应关系.FOD可诱导CNE1细胞凋亡,存在剂量-效应关系和时间-效应关系.结论:FOD对鼻咽癌细胞株CNE1有显著的抑制增殖作用,作用机制可能与诱导细胞凋亡有关.%Objective: To investigate the effects of the total flavones of oldenlandia diffusa willd (FOD) on the proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE1. Methods; CNE1 cells were treated with a series of doses of FOD for 24 h or 48 h. The cellular growth inhibitory potency and cellular apoptosis rate were assessed by CCK-8 assay and Annexin V-FITC apoptotic detection assay, respectively. Results; FOD inhibited proliferation of CNE1 cells in a dose-dependent and time-dependent manner. Icjq of 24 h and 48 h were (27. 0 ± 2.5) mg/L and (12. 1 ±1.3) mg/L, respectively. Furthermore, FOD induced apoptosis of CNE1 cells in a dose-dependent and time-dependent manner. Conclusion; FOD inhibits the proliferation of nasopharyngeal carcinoma cell line CNE1, which is suggested to be involved in the induction of apoptosis.