新疆哈萨克族、汉族RA患者HLA-DR4等位基因及其与疾病活动的关联性研究

摘要

目的:探讨新疆哈萨克族、汉族类风湿关节炎(RA)患者的遗传易感基因的特点和其与临床及实验室指标的关系.方法:使用PCR-SSP方法检测新疆哈萨克族、汉族RA患者和健康者HLA-DR4的基因频率,分析其与RA临床、实验室指标之间的关系.结果:HLA-DR4等位基因在哈萨克族、汉族RA患者中出现频率均高于同族HC组(P<0.05或0.01),哈萨克族RA患者HLA-DR4阳性率高于汉族RA患者(P<0.01).哈萨克族、汉族RA患者DR4阳性与DR4阴性患者发病年龄、病程、肿痛关节数、DAS28评分、ESR、CRP、RF、抗环瓜氯酸肽抗体、抗角蛋白抗体未发现有差异.哈萨克族HLA-DR4阳性患者出现早期骨破坏的发生率高于HLA-DR4阴性组(P<0.01).结论:HLADR4是新疆哈萨克族、汉族RA患者主要的易感基因,哈萨克族患者中HLA-DR4阳性与骨破坏有关.%Objective:To evaluate the correlations between HLA-DR4 gene and clinical characteristics or laboratory parameters of rheumatoid arthritis (RA) in Kazaks and Han population in Xinjiang. Methods:The rnfrequency of HLA-DR4 allele was determined in 122 Kazaks RA patients and 103 controls using polymerase chain reaction sequence-specific (PCR-SSP);so was in 115 Han patients and 119 controls. Results:HLA-DR4 allele frequency was significantly higher in RA patients in both Kazaks and Han population , while it was also significant higher in Kazaks patients that in Han patients (P <0. 01). There was no significant difference in onset time , disease duration , joint swelling counts , joint pain counts, DAS28 scores, ESR, CRP, RF, CCP or AKA between DR4 positive and negative patients. However, there were significantly higher bone erosion rate on X-ray in DR4 positive Kazaks patients (P<0. 01). Conclusions:HLA-DR4 is correlated with RA in both Kazaks and Han population in Xinjiang. Bone erosion is more frequent in DR4 positive patients in Kazaks population.