纳米羟基磷灰石对卵巢癌细胞株的抑制作用研究

摘要

Objective To investigate the effect and mechanism of nanoscale hydroxyapatite (nHAP) upon the growth of ovarian cancer cell line SKOV3.Methods The ovarian cancer cell line SKOV3 was cultured.The 30％-,50％-and 70％-inhibitory concentrations (IC30,IC50 and IC70) of nHAP upon the ovarian cancer cell line SKOV3 were calculated,and the dose-effect and time-effect curves were delineated.The SK-OV3 cells treated with IC50 of nHAP and those untreated with nHAP were prepared for transmission electron microscopic observation and flow cytometry detection.Results After nHAP treatment for 48 h,different concentrations of nHAP could inhibit the growth of ovarian cancer SKOV3 cells.The IC30,IC50 and IC70 of ovarian cancer SKOV3 cells were calculated as 9.46,28.83 and 60.80 mg/L,respectively.During the 48-h period of different concentrations of nHAP treatment,the inhibitory rate was elevated over time and remained slow and stable after 48 h.Morphological observation and quantitative analysis demonstrated that 48-h treatment with IC50 of nHAP could induce the apoptosis of ovarian cancer SKOV3 cells with a apoptosis rate of (43.32 ± 12.53) ％,significantly higher compared with (10.88 ± 5.48) ％ in the control group (P ＜ 0.05).Compared with the control group,the percentage of SKOV3 cells at G0/G1 phase was significantly higher,whereas the proportion of SKOV3 cells at S phase was considerably lower after treatment with IC5o of nHAP (both P ＜ 0.05).Conclusions nHAP exerts significant effect upon suppressing the growth of ovarian cancer cell line SKOV3.The underlying mechanism is probably that nHAP affects the ovarian cancer SKOV3 cells during the S phase and induces the apoptosis of cancer cells.%目的 研究纳米羟基磷灰石(nHAP)对卵巢癌细胞株SKOV3生长的影响及其作用机制.方法 培养卵巢癌SKOV3细胞,分别计算出nHAP对卵巢癌SKOV3细胞的30％、50％、70％抑制浓度(IC30、IC50、IC70),绘制量效曲线和时效曲线.选用IC50的nHAP作用卵巢癌SKOV3细胞48 h,与无nHAP的对照组卵巢癌SKOV3细胞对比,分别进行透射电镜观察、流式细胞仪检测.结果 nHAP作用48 h后,不同浓度nHAP对卵巢癌SKOV3细胞均表现出抑制作用,nHAP对卵巢癌SKOV3细胞的IC30、IC50、IC70分别为9.46、28.83、60.80 mg/L.IC30、IC50、IC70的nHAP作用于卵巢癌SK-OV3细胞48 h期间,抑制率随时间延长而升高,48 h后抑制率增速变缓.形态学观察和定量分析显示IC50的nHAP作用卵巢癌SKOV3细胞48 h可诱导其凋亡,其凋亡率为(43.32±12.53)％,对照组为(10.88±5.48)％,组间比较差异有统计学意义(P＜0.05),且与对照组比较,IC50的nHAP作用下的卵巢癌SKOV3细胞G0/G1期百分比高、S期细胞百分比低(P均＜0.05).结论 nHAP对卵巢癌细胞株SKOV3的生长具有明显的抑制作用,其机制可能是nHAP作用于卵巢癌SKOV3细胞的S期,诱导肿瘤细胞凋亡.