消幻汤对精神分裂症大鼠模型自主活动及纹状体内多巴胺含量的影响

摘要

Objective To investigate the effects of Xiaohuan Decoction on the dopamine in striatum and locomotor activity of schizophrenia model,and to explore the possible mechanism of Xiaohuan Decoction in treatment of schizophrenia. Methods Fifty — two rats were randomly assigned into four groups: normal group.model group.Xianhuan Decoction group,Risperidone group＜each group contained thirteen rats. All rats were injected intrapertoneally with 0. 1 mg/kg MK801 for 14 days to establish schizophrenia model. Following that, Xianhuan Decoction group was fed with the drug 4 ml/kg Once a day continuously for 4 wks. Risperidone group was fed with 0. 2 mg ? (4ml ? kg)"1. We use the stereotyped behavior test.open field test and electrochemical coulometric array HPLC determination to detect redefined parameters. Results (l)Compared with the normal group,the effect of Xiaohuan Decoction on reduced the stereotyped behavior (P ＜ 0. 05) in rat model of schizophrenia was a8 equal as risperidone ; (2)Total cross—grid was (243.75±86. 82) in Xianhuan Decoction group,(242. 10±S9. 56) in Risperidone group, (315.08±65. 93) in model group. There was a significant difference between the two groups and model group (P＜ 0. 05). (3)Compared to the model group, the levels of DA and DOPA in Xianhuan Decoction group and Risperidone group were significantly decreased (P＜0. 05), The content of HVA in Xianhuan Decoction group was significant decreased compared to the model group (.P ＜. 0.05). hut no significant difference between Risperidone group and model group (P ＞ 0. 05). Conclusions Xianhuan Decoction can improve cognitive impairment and improve locomotor activity. The effect is comparable to Risperidone. It regulates the content of DA and DA metabolites in the striatum to achieve the treatment effect on schizophrenia.%目的 通过研究消幻汤对精神分裂症动物模型自主活动及纹状体内多巴胺及其代谢产物含量的影响,探讨消幻汤治疗精神分裂症的可能机制.方法 雌性Sprague Dawley大鼠,分为:正常组、模型组、消幻汤组、利培酮组,每组13只.腹腔0.1 mg/kg的MK801连续注射14d制作模型后,分别给予相应治疗.应用刻板行为评分、旷场实验及库仑阵列电化学高效液相色谱法测定相应指标.结果 (1)与正常组比较,消幻汤可以有效抑制精神分裂症大鼠模型刻板行为(P＜0.05),与利培酮组效果相当；(2)给药后消幻汤组(243.75±86.82)格及利培酮组(242.10±89.56)格,自主活动与模型组(315.08±65.93)格相比,差异有统计学意义(P＜0.05);(3)神经递质化验结果显示:消幻汤组与利培酮组,多巴胺及二羟基苯乙酸水平与模型组比较含量明显减低(P＜0.05);消幻汤组高香草酸含量与比模型组显著降低(P＜0.05),而利培酮组与模型组比较差异无统计学意义(P＞0.05).结论 消幻汤可以增加精神分裂症模型大鼠的自主活动并改善认知损害,疗效与利培酮相当；消幻汤可以调节纹状体内多巴胺及其代谢产物含量,从而达到治疗精神分裂症的作用.