急性冠脉综合征患者基质金属蛋白酶-9水平及与炎症因子相关性研究

摘要

Objective To investigate the correlation between levels of matrix metalloproteinase-9 (MMP-9) in patients with acute coronary syndrome and inflammatory cytokines .Methods One hundred and twenty cases were collected clinically and divided in-to 4 groups, each consisting of 30 cases: the acute myocardial infarction group (the AMI group), the unstable angina group (the UA group), the stable angina group(the SA group), the control group, coronary imaging did not reveal significant coronary stenosis ).Lev-els of MMP-9, TNF-α, IL-6, IL-1βwere detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA).Results For the normal control group , significant differences could be noted in the levels of MMP-9, TNF-α, IL-6 and IL-1β, as compared with those of the AMI and UA groups(P<0.05), while for the SA group, marked differences could be noted in the levels of MMP-9, TNF-α, IL-6 and IL-1β, as compared with those of the AMI and UA groups (P<0.05).For the patients of the AMI group and UA group, there was obvious correlation between the level of MMP-9 and the levels of TNF-αand IL-6 ( r=0.37, 0.42, P<0.05; r=0.45, 0.51, P<0.05);weak correlation was shown between the level of MMP-9 and the level IL-1β(r=0.236, 0.259, P<0.05);and no correlation could be noted between the level of MMP-9 and the levels of IL-6 and IL-1β, when comparisons were made between the SA group and the control group .Conclusion Serum level of MMP-9 in patients with acute coronary syndrome was increased , ac-companied by the increased levels of TNF-α, IL-6 and IL-1β, and the increased level of MMP-9 was positively correlated with the levels of TNF-α, IL-6 and IL-1β.Increased levels of MMP-9, TNF-α, IL -6 and IL-1βimplied instability of the atherosclerotic plaque and could be an indicator for the prediction of acute coronary syndrome .%目的研究急性冠脉综合征患者基质金属蛋白酶-9（MMP-9）水平及其与炎症因子的相关性。方法临床收集病例及血液标本120例，试验分为4组，每组30例，分别为：急性心肌梗死组（ AMI组），不稳定型心绞痛组（ UA组），稳定型心绞痛组（ SA组），对照组。对照组为同期冠脉造影显示无明显局限性狭窄者。采用双抗夹心酶联免疫吸附（ ELISA ）法测定MMP-9、肿瘤坏死因子（ TNF）-α、白细胞介素（ IL）-6、IL-1β水平。结果正常对照组与AMI组、UAP组比较MMP-9、TNF-α、IL-6、IL-1β水平差异均有统计学意义（P＜0．05）。 SAP组与AMI组、UAP组比较MMP-9、TNF-α、IL-6、IL-1β水平差异均有统计学意义（P＜0．05）。 AMI组和UAP组MMP-9与TNF-α、IL-6显著相关（r＝0．37，0．42，P＜0．05；r＝0．45，0．51，P＜0．05），与IL-1β呈弱相关性（r＝0．236，0．259，P＜0．05）；而SAP组和对照组MMP-9与IL-6、IL-1β无相关性（P＞0．05）。结论急性冠脉综合征患者血清MMP-9增高，并伴有TNF-α、IL-6、IL-1β水平的增高，且血清MMP-9增高的水平与TNF-α、IL-6、IL-1β水平相关；MMP-9及TNF-α、IL-6、IL-1β水平升高提示了粥样斑块的不稳定性，有可能作为急性冠脉综合征的预测指标之一。