肝细胞肝癌组织中B细胞淋巴瘤因子9的表达及临床意义

摘要

目的：探讨B细胞淋巴瘤因子9(B-cell lymphoma 9, BCL9)在肝细胞肝癌(hepatocellular carcinoma, HCC)组织中的表达情况及临床意义。方法：采用Western Blot法检测8对新鲜冰冻HCC和对应癌旁组织中BCL9的蛋白表达；采用免疫组织化学法检测100例HCC和对应癌旁组织石蜡切片中BCL9的表达情况，并分析HCC中BCL9的表达与临床病理参数之间的关系。结果：Western Blot结果显示BCL9在HCC中的表达显著高于对应癌旁组织；免疫组化结果显示BCL9在HCC中主要定位于细胞核和细胞质，呈高表达，而在对应癌旁组织中主要定位于细胞质，表达较低或缺失；BCL9在HCC中的表达与肿瘤的分化程度和淋巴侵犯相关(P＜0.05)，与患者的性别、年龄、肿瘤直径、包膜完整、门静脉癌栓、血清HBsAg、甲胎蛋白及肝硬化无关(P＞0.05)。结论：BCL9在HCC中高表达，可能参与了HCC的发生、发展及转移。%Objective:To investigate the expression and clinical significance of B-cell lymphoma 9(BCLP) in hepatocellular carcinoma(HCC). Methods:Western Blot was performed to detect the protein expression of BCL9 in 8 pairs of HCC tissues and adjacent nontumorous tissues. Immunohistochemistry technique was used to analyze the expression of BCL9 in 100 cases of HCC and adjacent nontumorous tissues. The relationship between BCL9 and clinical pathology parameters were analyzed statistically. Results:Western Blot results showed that the expression of BCL9 was observably higher in HCC than that in adjacent nontumorous tissues. Immunohistochemistry analysis showed that BCL9 in HCC mainly located in nucleus and cytoplasm, and it showed high expression there, while in the adjacent nontumorous tissue it was mainly located in the cytoplasm , where the expression was low or absent . The expression of BCL9 was significantly correlated with tumor differentiation grade and lymphatic invasion(P<0.05), but had no association with patients’ gender, age, tumor diameter, intact tumor capsules, portal vein tumor thrombus, serum HBsAg, alpha fetoprotein or cirrhosis of liver (P>0.05). Conclusion: BCL9 was overexpressed in HCC, and may involved in the occurrence, development and metastasis of HCC.