抗氧化营养素对丙烯酰胺所致雄性大鼠生殖氧化损伤的保护作用

摘要

目的:探讨某些抗氧化营养素对丙烯酰胺(acrylamide,AA)所致雄性大鼠生殖氧化损伤的保护作用,为预防AA对人体的生殖危害提供一定科学依据.方法:选择SD雄性大鼠72只随机分成阴性对照组、阳性染毒组、VitE组、VitC组、硒组、VitE+硒组.染毒4周后处死大鼠,收集血清、肝脏、睾丸和附睾.测定血清中AA和环氧丙酰胺(glycidamide,GA)含量,血清、睾丸中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽S转移酶(GSH-ST)的含量,肝微粒体细胞色素P450 2E1活性.结果:阳性染毒组与阴性对照组相比体重、肝脏系数和附睾系数均减小,血清SOD、GSH-ST水平和睾丸MDA水平差异显著；而抗氧化营养素各组与阳性染毒组相比血清SOD、GSH-ST水平和睾丸MDA水平差异显著；VitE+硒组睾丸MDA水平与VitE、硒组差异显著；肝微粒体细胞色素P450 2E1活性VitE、VitC组与阳性染毒组差异显著.结论:抗氧化营养素对AA所致雄性大鼠生殖氧化损伤具有一定的保护作用,VitE和硒联合使用的保护效果更好.%Objective:To evaluate antioxidant nutrients' protection on reproductive oxidative damage male rats induced by acry lamide (AA). Methods:Six-week-old SD male rats were randomly divided into 6 groups. The six groups were negative control group, positive exposure group,VitE group,VitC group,selenium group and VitE+selenium group,respectively. After four weeks,rats were killed to collect serum,liver, testis,epididymis,and then the contents of AA,glycidamide (GA),malonaldehyde (MDA) ,superoxide dismutase (SOD) ,glutathione-S-transferase(GSH-ST) and liver microsomal cytochrome P450 2E1 were determined. Results: Rats ex posed to AA had significantly decreased weight,liver index and epididymis coefficient,and the SOD,GSH-ST levels in serum and the MDA level in testis were significant different compared with the controls. Compared with the positive exposure groups,the SOD,GSH ST levels in serum and the MDA level in testis of the antioxidant nutrients groups were significant different. Compared with the VitE, selenium groups,the MDA levels in testis of VitE+Se group had significant differences. The activity of liver microsomal cytochrome P450 2E1 in VitE and VitC groups had a statistical difference compared with the positive exposure group. Conclusion: The antioxi dant nutrients had a protective effect on male reproductive oxidative damage in rats induced by AA,and VitE+selenium protective ef fect of better use.