首页> 中文期刊> 《南昌大学学报(医学版)》 >成品次氯酸钠对粪肠球菌抗菌效果的体外研究

成品次氯酸钠对粪肠球菌抗菌效果的体外研究

         

摘要

Objective To compare antibacterial effect of the off-the-shelf sodium hypochlorite and freshly prepared sodium hypochlorite on enterococcus faecalis in vitro. Methods The paper diffusion method was used to evaluate the sensitivity of enterococcus faecalis to the off-the-shelf and freshly prepared sodium hypochlorite. Fifty papers were randomly and evenly divided into five groups;group A (1% freshly prepared sodium hypochlorite),group B (3% freshly prepared sodium hypochlorite) ,group C (1% off-the-shelf sodium hypochlorite) ,group D (3% off-the-shelf sodium hypochlorite) ,and group E (0. 9% saline). Diameters of antibacterial zone were measured in all groups. Results Antibacterial zone was observed in group A,B,C and D,but not in group E. There were no significant differences between group A and group das well as between group B and group D(all P>0. 05). While there were significant differences between group A and group B,as well as between group C and group D(all P<0. 01). Group A,B,C and D were all significantly more effective than group E(all P<0. 01). Conclusion The off-the-shelf sodium hypochlorite solutions have the same antibacterial effect as freshly prepared sodium hypochlorite solutions on enterococcus faecalis,and 3% sodium hypochlorite is significantly more effective than 1% sodium hypochlorite.%目的 体外比较成品和新鲜配制的次氯酸钠对粪肠球菌的抗菌效果.方法 采用纸片扩散法药敏实验,将50片无菌纸片按随机数字表法分为5组:分别观察新鲜配制的1%次氯酸钠溶液(A组,n=10)、新鲜配制的3%次氯酸钠溶液(B组,n=10)、成品的1%次氯酸钠溶液(C组,n=10)、成品的3%次氯酸钠溶液(D组,n=10)和生理盐水(E组,n=10)对粪肠球菌的抗菌效果,并测量其抑菌圈直径的大小(mm).结果 除E组外,A、B、C、D组均有抑菌圈形成;A组与C组、B组与D组抑菌圈直径差异无统计学意义(均P>0.05);A组与B组、C组与D组抑菌圈直径差异有统计学意义(均P<0.01);E组与A、B、C、D组抑菌圈直径差异均有统计学意义(均P<0.01).结论 成品次氯酸钠溶液和新鲜配制的次氯酸钠溶液对粪肠球菌的抗菌效果相同,3%次氯酸钠溶液对粪肠球菌的抗菌效果优于1%次氯酸钠溶液.

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