目的 探讨Er:YAG激光备洞对牙髓的生物学效应.方法 雄性SD大鼠66只随机分为实验组与对照组,实验组大鼠左上第一磨牙用高速牙钻备洞,右上第一磨牙用Er:YAG激光备洞,对照组大鼠磨牙不作任何处理.于不同时段分批处死大鼠,取标本做HE染色进行牙髓组织病理学观察,免疫组化染色法观察牙髓内降钙素基因相关肽(calcitonin cene-related peptide,CGRP)的表达.结果 Er:YAG激光备洞后牙髓组织病理学改变及降钙素基因相关肽的表达改变恢复正常要快于高速牙钻备洞.结论 Er:YAG激光备洞对牙髓的刺激小.%Objective To explore the biological effect of EnYAG laser using for cavity preparation. Methods Sixty-six SD male rats were randomly divided into experimental group and control group. Cavities on each experimental rat's upper left first molar were prepared by high-speed drill and cavities on the upper right first molar were prepared by EnYAG laser. Rats in control group were without any treatment. The animals were sacrificed at the scheduled time. Pathological changes in the dental pulp were observed by HE staining and the expression of CGRP were assayed by immunohistochemistry. Results Compared with those in high-speed drill group, it took less time to restore to normal for the expression of CGRP and the pathological appearance in the dental pulp in EnYAG laser group. Conclusion EnYAG laser for cavity preparation results in less stimulation to the dental pulp.
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