人前列腺癌细胞系DU145中肿瘤干细胞样侧群细胞的分离

摘要

Objective;To identify the side population (SP) cells from human prostate cancer cell line DU 145 and characterize DU 145 SP cells preliminary. Methods: Fluorescence activated cell sorting (FACS) was applied to isolate SP cells from DU 145 cell line. The isolated SP cells were cultured in serum - free medium to observe the growth pattern. Reverse transcription - polymerase chain reaction (RT - PCR) was used to detect the expression of ABCG2 in DU 145 SP cells and parental DU 145 cells. Results: SP cells were isolated from DU 145 cells by flow cytometry, with a proportion of 1.1%. When cultured in serum -free medium, SP cells showed a clustered growth pattern compared to the parental DU 145 cells. Furthermore, a higher expression of ABCG2 in SP cells was detected by RT -PCR in contrast to parental DU 145 cells. Conclusion; DU 145 cell line contained SP cells with cancer stem cell properties.%目的:分离人前列腺癌细胞系DU 145中的侧群(side population, SP)细胞,并初步分析其生物学特性.方法:采用荧光激活细胞分类(fluorescence activated cell sorting,FACS)技术,从DU 145细胞中分离出侧群细胞,并检测其比例;继而培养于无血清培养基中,观察其生长特性.采用反转录聚合酶链反应( RT-PCR)技术检测侧群细胞中ABCG2的表达水平.结果:DU 145细胞中存在含量极少的侧群细胞,比例约1.1％;培养于无血清培养基中成簇生长.和对应的母系DU 145细胞相比,DU 145侧群细胞的ABCG2表达增高.结论:人前列腺癌细胞系DU 145中存在具有肿瘤干细胞特性的侧群细胞.