hsa - miR -136和 PPP2R2A 在骨巨细胞瘤组织中的表达及其意义

摘要

目的：检测 hsa - miR -136和 PPP2R2A 在骨巨细胞瘤组织中含量，并预测二者的作用位点。方法：采用 Real - time PCR 方法检测 hsa - miR -136和 Western - blot 方法检测 PPP2R2A 在骨巨细胞瘤组织和瘤旁组织含量。利用 miRNA 靶基因预测软件 TargetScan，对 hsa - miR -136的靶基因进行预测，BibiServ 软件分析hsa - miR -136与靶基因结合的自由能。结果：hsa - miR -136在骨巨细胞瘤组织中的含量明显高于瘤旁组织（P ＜0.05）。PPP2R2A 在骨巨细胞瘤组织中的含量明显低于瘤旁组织（ P ＜0.05）。hsa - miR -136和PPP2R2A 与骨巨细胞瘤患者的病理特征没有明显相关性。Kaplan - Meier 生存曲线分析显示 hsa - miR -136和 PPP2R2A 与骨巨细胞瘤患者生存时间具有显著相关性（P ＜0.05）。结论：hsa - miR -136和 PPP2R2A 在骨巨细胞瘤组织中表达异常，提示 hsa - miR -136和 PPP2R2A 可能与骨巨细胞瘤的发生、发展有密切关系。%Objective:To determine the levels of hsa - miR - 136 and PPP2R2A in giant cell tumor of bone. And binding sites of hsa - miR - 136 and PPP2R2A were predicated. Methods:The levels of hsa - miR - 136 and PPP2R2A in giant cell tumor of bone were detected by using Real - time PCR and Western - blot,respectively. The candidate target genes of hsa - miR - 136 were predicated by using TargetScan software. The minimum free energy predicted for hybridization was determined by BibiServ analysis. Results:The levels of hsa - miR - 136 were higher in cancer tissues than that in normal tissues(P < 0. 05). The levels of PPP2R2A were lower in cancer tissues than that in normal tissues(P < 0. 05). The levels of hsa - miR - 136 and PPP2R2A were not related with the pathological fea-ture of the patients with giant cell tumor of bone. Both hsa - miR - 136 and PPP2R2A were associated with the surviv-al rate of the patients with giant cell tumor of bone by using Kaplan - Meier survival curve analysis(P < 0. 05). Con-clusion:Abnormal hsa - miR - 136 and PPP2R2A were observed in giant cell tumor of bone cancer tissues,which suggest that there was association between hsa - miR - 136 and PPP2R2A and the development of giant cell tumor of bone.