Objective:The study was designed to evaluate the effection of methylselenocysteine(MSC)on antitu-mor and to delineate the underlying mechanism associated with observed in vitro between MSC and selenium -binding protein 1(SBP1)in gastric carcinoma cells SGC7901.Methods:Cells were treated with different concentrations and schedules of MSC.The growth and proliferation rate were assessed with tetrazolium blue micro -ELISA assay(MTT). The expression of SBP1 mRNA and protein were measured with quantitative Real -time PCR and Western blot analy-ses,respectively.Results:Antiproliferative activities were expressed as drug concentrations that induced inhibition of cell growth compared with cell growth of untreated controls.Quantitative Real -time PCR and Western blot analysis indicated that MSC induced a statistically significant increase in SBP1 mRNA and protein expression as compared to the controls in most experiment groups except the 25μmol/L group in Real -time PCR analysis.Conclusion:Inhibi-ting cell proliferation is not the main mechanism of anticancer action of MSC in gastric carcinoma cells SGC7901.It was demonstrated that following the increasing of MSC concentration,SBP1 mRNA and protein expression were in-creased,which reversed the downregulation of SBP1 in gastric carcinoma cells SGC7901.These results provide evi-dence for a link between SBP1 and the mechanism of MSC on anticancer effection.%目的：研究甲基硒代半胱氨酸（MSC）对胃癌 SGC7901细胞增殖的抑制作用，并寻找这种抑制作用与硒结合蛋白1（SBP1）之间的关系。方法：用不同浓度 MSC 处理胃癌 SGC7901细胞，MTT 法检测胃癌 SGC7901细胞生长抑制率，实时荧光定量 PCR（Real －time PCR）和免疫印迹（Western blot）技术分别检测 SBP1 mRNA和蛋白水平的表达。结果：与对照组相比，MTT 检测结果显示胃癌 SGC7901细胞经浓度为25、50、75、100μmol／L 的 MSC 处理后，体外增殖均受到抑制。Real －time PCR 结果显示，25μmol／L 浓度组 MSC 对 SBP1 mRNA 表达影响无明显统计学意义；但50、75、100μmol／L 浓度组 SBP1 mRNA 的表达均明显上调，在 MSC 有效浓度范围之内，随着 MSC 浓度的梯度增加，SBP1 mRNA 的表达也相应上调。Western blot 结果显示 MSC 分别作用24h、48h 及72h 后，各浓度组之间 SBP1蛋白的相对表达量比较差异均有统计学意义，且随着 MSC 浓度的梯度增加，SBP1蛋白的相对表达量呈上升趋势。结论：MSC 是一种低毒的含硒抗癌活性物质，它在干预胃癌中的作用并非基于对肿瘤细胞的直接毒性效应。MSC 可能通过上调胃癌 SGC7901细胞 SBP1的表达而发挥抗癌作用。
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